We narrowed to 36,680 results for: kan

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pENTR_hEMSY

Plasmid

#125156

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

EMSY (EMSY Human)

Use

Gateway Cloning

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV812

Plasmid

#126605

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Article

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV809

Plasmid

#126602

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Article

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-KIF17*-strep

Plasmid

#120167

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 17 (KIF17 Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF17: 1-509 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1

Plasmid

#121831

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

x-dCas9(3.7)/LSD1 (open)

Use

Gateway Cloning and Synthetic Biology

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1A(sfGFP)

Plasmid

#126960

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSB161 - pL2_pSB90_2x35S::GUS::tMAS

Plasmid

#123197

Purpose

binary plant vector for transient expression of GUS (with introns)

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::GUS::tMAS

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET_MBP-RGG-GFP-RGG

Plasmid

#124949

Purpose

Expression of MBP-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

MBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

EGFP, His, and MBP

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-gcry-TagBFP

Plasmid

#117777

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1

Plasmid

#124919

Purpose

For expression of wild-type SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA(1,20,6)-ADAR2(E488Q)

Plasmid

#124621

Purpose

Expresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

ADAR2(E488Q)

Use

AAV

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NLS

Plasmid

#124626

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD(E1008Q)_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD (E1008Q)_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

E1008Q hyperactive mutant

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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Lamin A (R435C)-mRFP

Plasmid

#124274

Purpose

Lamin A mutant (R435C), C-terminally tagged with mRFP. Mutation associated with Progeria.

Depositor

Eric Schirmer

Insert

LMNA (R435C) (LMNA Human)

Tags

mRFP

Expression

Mammalian

Mutation

LMNA (R435C)

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pKAd5f11pES-PmeI

Plasmid

#122698

Purpose

To generate an E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and the exogenous gene expression cassette.

Depositor

Zhuo-Zhuang Lu

Article

Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

Type

Empty backbone

Use

Adenoviral

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pXWJ109Hg-gfp

Plasmid

#123148

Purpose

A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, PmerT

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCoofy61

Plasmid

#122013

Purpose

E.coli vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site

Depositor

Peggy Stolt-Bergner, Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-eGFP

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-PSAT

Plasmid

#74115

Purpose

Expression of PSAT

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

pET28a-PSAT (PSAT1 Human)

Expression

Bacterial and Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEG302 5aa SunTag VP64 nog

Plasmid

#115480

Purpose

CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 17, 2019

Availability

Academic Institutions and Nonprofits only

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p8xEGFP-N1

Plasmid

#122168

Purpose

Expression of 8xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

8xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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p3xEGFP-N1

Plasmid

#122163

Purpose

Expression of 3xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

3xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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