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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,673 results for: yeast

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Showing: 9221 - 9240 of 10673 results

  3. L284F Cus2p

    Plasmid
    #73059
    Purpose
    S. cerevisiae Cus2p gene with L284F Mutation
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Leucine 284 to Phenylalanine
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    73059-plasmid-map-sequence-id-150795

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  4. D282N Cus2p

    Plasmid
    #73060
    Purpose
    S. cerevisiae Cus2p gene with D282N Mutation
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Asp 282 to Asn
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    73060-plasmid-map-sequence-id-150798

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  11. pT22-GluCNZ-6His

    Plasmid
    #213504
    Purpose
    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.
    Depositor
    John Denu
    Article
    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12
    Insert
    Glucanase (glucan endo-1,3-beta-glucosidase)
    Tags
    6His tag
    Expression
    Bacterial
    Mutation
    1-36 Signal Sequence Deletion
    Promoter
    T7
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213504-plasmid-map-sequence-id-424932

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  13. pJY3948

    Plasmid
    #113338
    Purpose
    6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression
    Depositor
    Suzanne Sandmeyer
    Article
    Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.
    Insert
    2-pyrone synthase, codon optimized for Y. lipolytica
    Tags
    6XHis
    Expression
    Yeast
    Mutation
    Please see depositor comments
    Available Since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113338-plasmid-map-sequence-id-218737

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  14. pADH99

    Plasmid
    #90979
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    US_HIS1
    FRT
    pENO1
    C. albicans Cas9
    pMAL2
    FLP
    NAT 1 of 2
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90979-plasmid-map-sequence-id-180505

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Showing: 9221 - 9240 of 10673 results