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Str-KDEL_SBP-EGFP-LAP

Plasmid

#222322

Purpose

Synchronize the trafficking of LAP from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAP fused to SBP-EGFP (ACP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-EGFR

Plasmid

#222325

Purpose

Synchronize the trafficking of EGFR from the ER.

Depositor

Franck Perez

Article

Scharaw et al J Cell Biol. 2016 Nov 21;215(4):543-558.

Insert

Streptavidin-KDEL and EGFR fused to SBP-EGFP (EGFR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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DG SaCas9 puro V3

Plasmid

#226960

Purpose

CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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SBC009876

Plasmid

#226283

Purpose

Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

Depositor

Nigel Scrutton

Article

Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

Inserts

Coniferyl alcohol acyltransferase
Eugenol synthase 1

Expression

Bacterial

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-dSV40-CD80-mApple

Plasmid

#223595

Purpose

Lentiviral expression of human CD80 with mApple in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

mApple

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-dSV40-CD80-mGFP

Plasmid

#223592

Purpose

Lentiviral expression of human CD80 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

mGFP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD80 (I92R)

Plasmid

#223589

Purpose

Lentiviral expression of human CD80 (I92R) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Mutation

I92R

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CD80

Plasmid

#223581

Purpose

Protein purification of human CD80 with Twin-Strep and SNAP tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Tags

Twin-Strep, SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-TMEM173-ts1

Plasmid

#174283

Purpose

TMEM173 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

TMEM173 shRNA (STING1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-TMEM173-ts2

Plasmid

#174284

Purpose

TMEM173 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

TMEM173 shRNA (STING1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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