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pcDNA 3.2/V5-DEST NET1A S46A

Plasmid

#69818

Purpose

Expresses NET1A-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Tags

V5

Expression

Mammalian

Mutation

S46A

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP-WAF1-ES

Plasmid

#16449

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 stop codon mut (CDKN1A Human)

Expression

Mammalian

Mutation

stop codon at amino acid 49 instead of tryptophan…

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
FUW TetO NET1A-V5 S46A

Plasmid

#69829

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

S46A

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Expression

Bacterial and Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLN552 (FuGW-S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

Plasmid

#105202

Purpose

Module 1 - synthetic promoter USF1 drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad3

Plasmid

#89828

Purpose

Knockdown human Smad3 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD3 shRNA (SMAD3 Human)

Use

Retroviral

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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GoldyTALEN-ppp1cab TAL1

Plasmid

#41835

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

protein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-M100/125/147/213-siResist

Plasmid

#49858

Purpose

Encodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100, Methionine 125, Methionine 147, an…

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
SLX4-SFB-D556-637

Plasmid

#247860

Purpose

Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged SLX4 D556-637

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

SLX4 (SLX4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

deletion of amino acid 556-637

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab813-bGlobin_3xF-BRCA1(full-length)-Y1845D

Plasmid

#249024

Purpose

This plasmid expresses the full length BRCA1 sequence (Y1845D mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

Y1845D

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab814-bGlobin_3xF-BRCA1(full-length)-M1775R

Plasmid

#249025

Purpose

This plasmid expresses the full length BRCA1 sequence (M1775R mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

M1775R

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab816-bGlobin_3xF-BRCA1(full-length)-G1788V

Plasmid

#249026

Purpose

This plasmid expresses the full length BRCA1 sequence (G1788V mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

G1788V

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab818-bGlobin_3xF-BRCA1(full-length)-A1708E

Plasmid

#249027

Purpose

This plasmid expresses the full length BRCA1 sequence (A1708E mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

A1708E

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab819-bGlobin_3xF-BRCA1(full-length)-Y1845F

Plasmid

#249028

Purpose

This plasmid expresses the full length BRCA1 sequence (Y1845F mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

Y1845F

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab966-pUC19-HAL-BRCA1-BSD-P2A-2xHA-FKBP12-F36V-HAR-BRCA1

Plasmid

#249004

Purpose

This plasmid expresses the degron tag knock-in sequence at the N-terminus of the endogenous BRCA1 locus

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

FKBP12 (FKBP1A Human)

Use

CRISPR

Tags

2xHA

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pAvA139

Plasmid

#248145

Purpose

mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen1' mutations

Depositor

Emil Jensen

Article

van Aalst et al Commun Biol. 2025 Nov 27. doi: 10.1038/s

Insert

luxR (gen1 mutations)

Use

Integration vector

Tags

Gal4 activation domain and nuclear localization s…

Expression

Yeast

Mutation

Gal4_AD: N24K, P41 (CCA→CCG), N46D, T92S, V98 (GT…

Promoter

pPGK1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pSA143 Cas9-BFP with core cHS4 insulators

Plasmid

#249153

Purpose

Overexpression of SpCas9-BFP and blasticidin resistance gene. Flanked by cHS4 core insulators. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

Cas9-TagBFP with core cHS4 insulators

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pPD231 PPH-T2A-dCas9-NFZ-P2A-BFP

Plasmid

#249155

Purpose

Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

PPH-T2A-dCas9-NFZ-P2A-mTagBFP2

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-EGFP-fibrillarin-FRT

Plasmid

#247341

Purpose

FLP/FRT based mammalian expression vector for EGFP-fibrillarin driven by EF1α promoter.

Depositor

Kazuhiro Maeshima

Article

Imai et al Mol Biol Cell. 2017 Nov 7;28(23):3349-33

Insert

fibrillarin (FBL) (FBL Human)

Tags

EGFP

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL(N178/298/358Q)

Plasmid

#214804

Purpose

Mammalian expression of human MPL(N178/298/358Q)

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human MPL(N178/298/358Q) (MPL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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