Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 13,357 results for: BASE

Showing: 9281 - 9300 of 13357 results

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pET21-pelB-LaG94-18

Plasmid

#228972

Purpose

For bacterial expression of anti-GFP nanobody LaG94-18, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-18

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-2

Plasmid

#228975

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-2

Plasmid

#228962

Purpose

For bacterial expression of anti-GFP nanobody LaG94-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-1

Plasmid

#228961

Purpose

For bacterial expression of anti-GFP nanobody LaG94-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-44

Plasmid

#228981

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-44

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-45

Plasmid

#228982

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-45, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-45

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-10

Plasmid

#228978

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-10

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-4

Plasmid

#228964

Purpose

For bacterial expression of anti-GFP nanobody LaG94-4, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-5

Plasmid

#228987

Purpose

For bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-6

Plasmid

#228988

Purpose

For bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-6

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-8

Plasmid

#228976

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-9

Plasmid

#228977

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1-NbON10-NanoLuc

Plasmid

#198691

Purpose

Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, IgK leader sequence, NanoLuc, and twin…

Expression

Mammalian

Promoter

CMV promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pINQ-ON10

Plasmid

#198689

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, HA tag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pINQ-NbH4

Plasmid

#198690

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody H4

Tags

6xHis tag, Avitag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRGEB32-CrU6-2-35S

Plasmid

#226706

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from CaMV 35S promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrU6-2

Use

CRISPR

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_GR-RE_MLPmin_BC1093-luc2

Plasmid

#227113

Purpose

Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1093

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered GR response element linked to MLP minimal promoter driving barcode 1093 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRGEB32-CrActin-35S

Plasmid

#226708

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from CaMV 35S promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrActin

Use

CRISPR

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRGEB32-CrActin-UBI

Plasmid

#226709

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrActin

Use

CRISPR

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRGEB32-CrU6-2-UBI

Plasmid

#226707

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrU6-2

Use

CRISPR

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 9281 - 9300 of 13357 results

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