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mKO-Zyxin-6

Plasmid

#57853

Purpose

Localization: Focal Adhesions, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

Insert

Zyxin (ZYX Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-FYN-N-10

Plasmid

#55483

Purpose

Localization: Membrane Associated Kinase, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

FYN (FYN Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

Plasmid

#58490

Purpose

Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

Depositor

Adam Cohen

Article

Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

Insert

Archaerhodopsin-3 w/ D95H point mutation fused to eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Aspartic Acid 95 to Glutamine

Promoter

Ubiquitin

Available Since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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LV-CMV-Cer-FL- Bmal L95E

Plasmid

#47374

Purpose

Bmal full length with cer tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus

Expression

Mammalian

Mutation

L95E

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA/Bmal F423R/V435R/W427R

Plasmid

#47351

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

F423R/V435R/W427R

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN L113E

Plasmid

#47356

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L113E

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN F122D

Plasmid

#47357

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, F122D

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN W284A

Plasmid

#47358

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, W284A

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN V315R

Plasmid

#47359

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, V315R

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC L115E

Plasmid

#47377

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

L115E

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN L57E

Plasmid

#47354

Purpose

Clock fragment mutatation tagged with VenN

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L57E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (P1035S)

Plasmid

#29558

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed proline 1035 to serine

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (C638S)

Plasmid

#29555

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed cysteine 638 to serine

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
Neurl1Rm-Flag pBacPAK8-3

Plasmid

#17326

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Insect

Mutation

Cys 521 changed to Ser Cys 524 changed to Ser

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCRIS-PITChv2-dTAG-BSD (BRD4)

Plasmid

#91795

Purpose

PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

FKBP_F36V-2xHA-P2A-BSD

Use

CRISPR

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promotorless

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-dTAG-Puro (BRD4)

Plasmid

#91796

Purpose

PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

FKBP_F36V-2xHA-P2A-Puro

Use

CRISPR

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promotorless

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-Puro-dTAG (BRD4)

Plasmid

#91793

Purpose

PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

Puro-P2A-2xHA-FKBP_F36V

Use

CRISPR

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promotorless

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-BSD-dTAG (BRD4)

Plasmid

#91792

Purpose

PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

BSD-P2A-2xHA-FKBP_F36V

Use

CRISPR

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promoterless

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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R701-X65-527: His6-tev-Hs.CALM1(2-148)

Plasmid

#159693

Purpose

E. coli protein expression of His6-tev-Hs.CALM1(2-148)

Depositor

Dominic Esposito

Article

Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

Insert

His6-tev-Hs.CALM1(2-148) (CALM1 Human)

Tags

His6-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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BII-CMV-AfmW3A

Plasmid

#133402

Purpose

Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Afamin and Wnt3a (Afm, Wnt3a Mouse)

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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