We narrowed to 15,730 results for: NOL

1. SpCas9-NRCH-ABE8.17-m+V106W

   Plasmid

   #198551

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-ABE8.17-m+V106W
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-Flag-cMyc T58A

   Plasmid

   #20075

   Depositor

   Juan Belmonte

   Article

   Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

   Insert

   cMyc (MYC Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T58A

   Available Since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-hSyn-GACh2.0

   Plasmid

   #121921

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0

   Depositor

   Yulong Li

   Article

   Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

   Insert

   GPCR activation based ACh sensor GACh2.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKII-iGECI-NES

   Plasmid

   #160424

   Purpose

   AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI-NES
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. SFFV-SMASH-CTERM-GFP

   Plasmid

   #185766

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, SMASh

   Mutation

   none

   Promoter

   SFFV

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. B*57:03:01

   Plasmid

   #203260

   Purpose

   Overexpress HLA allele

   Depositor

   Derin Keskin, Catherine Wu

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   B*57:03:01 (HLA-B Human)
   

   Expression

   Mammalian

   Available Since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. SFFV-SMASH-NTERM-GFP

   Plasmid

   #185761

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, SMASh

   Mutation

   none

   Promoter

   SFFV

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SARS_COV2_ORF10_HA_BASU

   Plasmid

   #153980

   Purpose

   Express Orf10 gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf10 (ORF10 Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG

   Plasmid

   #206956

   Purpose

   AAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulins

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   mScarlet-I, MmMT3, QtEnc
   

   Use

   AAV

   Tags

   FLAG

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AID-BE4max-P2A-GFP

    Plasmid

    #157948

    Purpose

    Mammalian expression of AID-BE4max-P2A-GFP

    Depositor

    Dali Li

    Article

    Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    AID-BE4max
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. SFFV-AID-NTERM-GFP

    Plasmid

    #185763

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, AID

    Mutation

    none

    Promoter

    SFFV

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9 TadCBEa-V106W

    Plasmid

    #193838

    Purpose

    Express TadCBEa-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa V106W-SpCas9 D10A
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-hA3A-BE3-D131Y

    Plasmid

    #113424

    Purpose

    Expresses hA3A-BE3-D131Y in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-BE3-D131Y (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_D131Y

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCD4-TagRFP-T (RHPMP151)

    Plasmid

    #119238

    Purpose

    Expresses CD4 fused to TagRFP-T

    Depositor

    Ricardo Henriques

    Article

    Pereira et al Front Immunol. 2019 Apr 5;10:675. doi: 1

    Insert

    CD4 (CD4 Human)
    

    Tags

    tagRFP-T

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. non-targeting control gRNA (BRDN0001146145)

    Plasmid

    #80198

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_UdgX-APOBEC1-UdgX-nCas9

    Plasmid

    #163555

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-hA3H-BE3

    Plasmid

    #113416

    Purpose

    Expresses hA3H-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3H-BE3 (APOBEC3H Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1a_FL MUT TET1

    Plasmid

    #124081

    Purpose

    Expression of catalytic inactive form of full-length TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - full length, mutated (TET1 Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1672Y, D1674A

    Promoter

    EF1 alpha

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Circular 200,100 with interspersed loops (FANCC)

    Plasmid

    #180192

    Purpose

    AAV vector carrying a guide RNA targeting the human FANCC mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Circular 200,100 with interspersed loops (CTNNB1)

    Plasmid

    #180193

    Purpose

    AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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