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pDHt/sk-PE

Plasmid

#92126

Purpose

Expression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9-eGFP

Use

CRISPR; Trichoderma reesei expression

Tags

EGFP

Promoter

Ppdc

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDAS12124_PEAR-GFP-preedited

Plasmid

#177179

Purpose

PEAR-GFP control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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vsv-Sgo1-GFP

Plasmid

#108494

Purpose

expression of vsv-Sgo1-EGFP

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

Shugoshin 1 (SGO1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMP2463

Plasmid

#107774

Purpose

Expression of EGFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EGFP in pBBR1-mcs5

Tags

pLacZ-EGFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDHt/sk-CE

Plasmid

#92128

Purpose

Expression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9-eGFP

Use

CRISPR; Trichoderma reesei expression

Tags

EGFP

Promoter

Pcbh2

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z05

Plasmid

#120048

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-PS Intein

Plasmid

#242020

Purpose

AAV version of PS Intein palsmid.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

PS Intein

Use

Adenoviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCL1313

Plasmid

#217955

Purpose

Expression of human H3.1 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H3.1 (H3C1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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PECAM-H2B-GFP

Plasmid

#14689

Depositor

Victoria Bautch

Article

Zeng et al Blood. 2007 Feb 15. 109(4):1345-52.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Tags

H2B-GFP

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
Fluorescent reporter for MOBE1-2

Plasmid

#219945

Purpose

Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end

Use

CRISPR

Expression

Mammalian

Mutation

EGFP(A111V/L202S)

Promoter

CMV, U6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMP4657

Plasmid

#107773

Purpose

Expression of EGFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EGFP in pME6010

Tags

pLacZ-EGFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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Fluorescent reporter for MOBE3-4

Plasmid

#219946

Purpose

Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3

Use

CRISPR

Expression

Mammalian

Mutation

EGFP(A111V/L202S)

Promoter

CMV, U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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PECAM-H2B-GFP-Hygro

Plasmid

#14684

Depositor

Victoria Bautch

Article

Zeng et al Blood. 2007 Feb 15. 109(4):1345-52.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Tags

H2B-GFP and PGK-Hygro

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pLP-hSox2-ires-gfp

Plasmid

#49391

Purpose

mammalian bicistronic expression of human Sox2 and EGFP

Depositor

Randall McKinnon

Insert

Sox2 (SOX2 Human)

Use

Cre/Lox; Acceptor vector

Tags

6xHN, EGFP, and IRES

Expression

Mammalian

Mutation

TGA (stop) to CCA (Pro) at nt 1027 with respect t…

Promoter

CMV

Available Since

Dec. 2, 2013

Availability

Academic Institutions and Nonprofits only
AP1297‐1

Plasmid

#87247

Purpose

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

Depositor

Geraldine Seydoux

Article

Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

Insert

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

Tags

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pATI (pKD-G7)

Plasmid

#62686

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pATM (pKD-G11)

Plasmid

#62690

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419/tdTomato

Use

RNAi

Expression

Mammalian

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pZA46

Plasmid

#158524

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP

Tags

eGFP

Expression

Plant

Mutation

D481V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA47

Plasmid

#158525

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP

Tags

eGFP

Expression

Plant

Mutation

L17E

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA48

Plasmid

#158526

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP

Tags

eGFP

Expression

Plant

Mutation

L17E, D481V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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