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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,743 results for: emb

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Showing: 9361 - 9380 of 18743 results

  2. pChlr-2SE

    Plasmid
    #52144
    Purpose
    Encodes module 2 with amino acids 12S-16E for recognition of nt 7G
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 2
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pChlr-8CQ

    Plasmid
    #52171
    Purpose
    Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in module 8
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. DHRS4

    Plasmid
    #39071
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DHRS4 (DHRS4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. PSB-CMV-Puro ArfGAP1_ALPS1-mkate2-3NLS-P2A-EGFP-Sec61B

    Plasmid
    #250912
    Purpose
    Dual color expression of mKate2-3NLS tagged rat ARFGAP1 ALPS1 and EGFP tagged human Sec 61B in mammalian cell lines
    Depositor
    Philipp Niethammer
    Publication
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Sec61B (Arfgap1, SEC61B Rat, Human)
    Tags
    EGFP on Sec61B and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 196–234 only
    Promoter
    CMV
    Available since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250912-plasmid-map-sequence-id-498488

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  10. phNFL-Q332P

    Plasmid
    #132599
    Purpose
    Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E Q332P mutation
    Depositor
    Anthony Brown
    Publication
    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nefl (Nefl Human)
    Expression
    Mammalian
    Mutation
    995A>C;Gln332Pro
    Promoter
    CMV
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132599-plasmid-map-sequence-id-260979

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  11. prNFL-N98S-EGFP

    Plasmid
    #132600
    Purpose
    Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation
    Depositor
    Anthony Brown
    Publication
    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nefl (Nefl Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    293A>G; Asn98Ser
    Promoter
    CMV
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132600-plasmid-map-sequence-id-260031

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  13. pEGFP-mAV

    Plasmid
    #129472
    Purpose
    Mammalian expression of full-length mouse archvillin (Supervillin, isoform 2)
    Depositor
    Elizabeth Luna
    Publication
    Oh et al J Cell Sci. 2003 Jun 1. 116(Pt 11):2261-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Supervillin, isoform 2 (Svil Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    129472-plasmid-map-sequence-id-252745

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  19. TMED5-bio-His

    Plasmid
    #53338
    Purpose
    Expresses full-length Transmembrane emp24 domain-containing protein 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TMED5 (TMED5 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53338-plasmid-map-sequence-id-83068

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Showing: 9361 - 9380 of 18743 results