Skip to main content
Addgene
Showing: 921 - 940 of 1080 results

  16. pD649-HAsp-CNTN5-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157026
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CNTN5 (CNTN5 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157026-plasmid-map-sequence-id-303161

    Enlarge

  17. pD649-HAsp-EMB-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156680
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    EMB (EMB Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156680-plasmid-map-sequence-id-302469

    Enlarge

  18. pDR111_mannose_Klf4Gata-3

    Plasmid
    #188401
    Purpose
    Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Inserts
    Klf4 (Klf4 Mouse)
    Gata-3 (Gata3 Mouse)
    Use
    Tags
    PhoD secretion peptide
    Expression
    Bacterial
    Mutation
    See Depositor Comments Below
    Promoter
    D-mannose
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188401-plasmid-map-sequence-id-370467

    Enlarge

  19. BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

    Plasmid
    #136688
    Purpose
    Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.
    Depositor
    Colin Garvie
    Article
    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66
    Insert
    Beclin-1 (BECN1 Human)
    Use
    Tags
    Trigger Factor
    Expression
    Bacterial
    Mutation
    DNA optimized for E. coli expression w/ three mut…
    Promoter
    T7
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136688-plasmid-map-sequence-id-272126

    Enlarge

Showing: 921 - 940 of 1080 results