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pSS22-IRES-NLS-eGFP

Plasmid

#241837

Purpose

Part I of the Donor construct for UBE3A reporter

Depositor

Albert Keung

Article

Kelkar et al Front Cell Dev Biol. 2025 Aug 29;13:1665

Insert

eGFP

Use

Donor plasmid

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_H1-5

Plasmid

#223820

Purpose

Bacterial expression of SynPspA H1-5 with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

truncation of helix 0

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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JBL6423

Plasmid

#183860

Purpose

sfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoter

Depositor

Julius Lucks

Article

Berman et al RNA. 2023 Nov;29(11):1658-1672. doi: 10.

Insert

E. coli thiB TPP Riboswitch Incumbent Rescue A regulated sfGFP

Expression

Bacterial

Promoter

J23119 - Anderson Promoter

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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JBL6428

Plasmid

#183865

Purpose

sfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoter

Depositor

Julius Lucks

Article

Berman et al RNA. 2023 Nov;29(11):1658-1672. doi: 10.

Insert

E. coli thiB TPP Riboswitch Incumbent Rescue B regulated sfGFP

Expression

Bacterial

Promoter

J23119 - Anderson Promoter

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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JBL6420

Plasmid

#183857

Purpose

sfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoter

Depositor

Julius Lucks

Article

Berman et al RNA. 2023 Nov;29(11):1658-1672. doi: 10.

Insert

E. coli thiB TPP Riboswitch Rescue Invader A regulated sfGFP

Expression

Bacterial

Promoter

J23119 - Anderson Promoter

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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JBL6421

Plasmid

#183858

Purpose

sfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoter

Depositor

Julius Lucks

Article

Berman et al RNA. 2023 Nov;29(11):1658-1672. doi: 10.

Insert

E. coli thiB TPP Riboswitch Full Rescue A regulated sfGFP

Expression

Bacterial

Promoter

J23119 - Anderson Promoter

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pETA_21 tRNA genes

Plasmid

#242431

Purpose

Expresses 21 tRNAs. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site as well as a T7 terminator downstream of each tRNA gene.

Depositor

Sebastian Maerkl

Article

Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

Insert

21 tRNA genes

Expression

Bacterial

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA032

Plasmid

#240781

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA031

Plasmid

#240780

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA030

Plasmid

#240779

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_low - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA023

Plasmid

#240772

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA022

Plasmid

#240771

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA021

Plasmid

#240770

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_low - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA019

Plasmid

#240768

Purpose

Plasmid contains the triple selection cassette gfp -tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA pBR322-ROP PrhaBAD BCD_medium - gfp -tetApBR322-ROP- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA018

Plasmid

#240767

Purpose

Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA pBR322-ROP PrhaBAD BCD_low - gfp - tetApBR322-ROP- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA015

Plasmid

#240764

Purpose

Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA p15A PrhaBAD BCD_low - gfp - tetAp15A- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA014

Plasmid

#240763

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 PrhaBAD BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA013

Plasmid

#240762

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 PrhaBAD BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA012

Plasmid

#240761

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 PrhaBAD BCD_low - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA009

Plasmid

#240758

Purpose

Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA pBR322-ROP J23100 BCD_high - gfp - tetApBR322-ROP- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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