|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21789||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)2063
Mutationcontains 5' 1kb upstream UTR/promoter Q93A mutation in open reading frame 3' 400bp downstream UTR/terminator
Entrez GeneFUS3 (a.k.a. YBL016W, DAC2)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
There are several sequence discrepancies between depositor's sequence and Addgene sequence in the 5' UTR. The 5'UTR in the plasmid is from a W303-derived strain, and the SGD used S288C strain for genome sequencing, so the mismatches could be SNPs. This plasmid is functional and these mismatches do not affect the ORF.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pES1002 was a gift from Richard Yu (Addgene plasmid # 21789 ; http://n2t.net/addgene:21789 ; RRID:Addgene_21789)
For your References section:Negative feedback that improves information transmission in yeast signalling. Yu RC, Pesce CG, Colman-Lerner A, Lok L, Pincus D, Serra E, Holl M, Benjamin K, Gordon A, Brent R. Nature. 2008 Dec 11. 456(7223):755-61. 10.1038/nature07513 PubMed 19079053