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(Plasmid #21789)


Item Catalog # Description Quantity Price (USD)
Plasmid 21789 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
  • Mutation
    contains 5' 1kb upstream UTR/promoter Q93A mutation in open reading frame 3' 400bp downstream UTR/terminator
  • GenBank ID
  • Entrez Gene
    FUS3 (a.k.a. YBL016W, DAC2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Eduard Serra ([email protected])

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are several sequence discrepancies between depositor's sequence and Addgene sequence in the 5' UTR. The 5'UTR in the plasmid is from a W303-derived strain, and the SGD used S288C strain for genome sequencing, so the mismatches could be SNPs. This plasmid is functional and these mismatches do not affect the ORF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pES1002 was a gift from Richard Yu (Addgene plasmid # 21789 ; ; RRID:Addgene_21789)
  • For your References section:

    Negative feedback that improves information transmission in yeast signalling. Yu RC, Pesce CG, Colman-Lerner A, Lok L, Pincus D, Serra E, Holl M, Benjamin K, Gordon A, Brent R. Nature. 2008 Dec 11. 456(7223):755-61. 10.1038/nature07513 PubMed 19079053
Commonly requested with: