We narrowed to 16,198 results for: grna

1. pRDA355_sgCiPELO #2

   Plasmid

   #229020

   Purpose

   Expression of a CRISPRi doxycycline inducible guide targeting PELO

   Depositor

   Francisca Vazquez

   Article

   Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

   Insert

   PELO gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE GFP-Grin1 KI

   Plasmid

   #131485

   Purpose

   Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin1, Grin1 Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORANGE GFP-Camk2a KI

   Plasmid

   #131484

   Purpose

   Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Camk2a, Camk2a Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pORANGE Dlg4-GFP KI

   Plasmid

   #131477

   Purpose

   Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Dlg4, Dlg4 Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pORANGE GFP-Actb KI

   Plasmid

   #131479

   Purpose

   Endogenous tagging of βactin: N-terminal (amino acid position: D2)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Actb, Actb Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgAPC(A)

   Plasmid

   #85557

   Purpose

   Expresses sgRNA targeting human APC around codon I1417

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   APC, WNT signaling pathway regulator (APC Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pORANGE Gria1-GFP KI

   Plasmid

   #131489

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Gria1, Gria1 Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV-EF1aGFP2AKAT7-W

   Plasmid

   #159292

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 (KAT7 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10)

   Promoter

   human EF1a promoter

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

   Plasmid

   #165082

   Purpose

   gRNA 1 of pair B for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgTP53(R273)

    Plasmid

    #85561

    Purpose

    Expresses sgRNA targeting human TP53 around codon R273

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    tumor protein p53 (TP53 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pORANGE GFP-CACNA1A KI

    Plasmid

    #131480

    Purpose

    Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacna1a, Cacna1a Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE mEos3.2-Camk2a KI

    Plasmid

    #131493

    Purpose

    Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and mEos3.2 donor (Camk2a, Camk2a Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYUU

    Plasmid

    #159751

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXPR_023d-sgCiCh2-2

    Plasmid

    #202444

    Purpose

    Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    Chromosome 2 gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORANGE GFP-Arpc5 KI

    Plasmid

    #131503

    Purpose

    Endogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Arpc5, Arpc5 Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PEA1-Puro-HEK3 CTT ins

    Plasmid

    #171996

    Purpose

    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    

    Expression

    Mammalian

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgTP53(A)

    Plasmid

    #85559

    Purpose

    Expresses sgRNA targeting human TP53 around codon F109

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    tumor protein p53 (TP53 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pLentiCRISPRv2-NONO 40

    Plasmid

    #127653

    Purpose

    Knock-out of human NONO

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

    Insert

    NONO sgRNA (NONO Human)
    

    Use

    Lentiviral

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCRISPRv2-NONO 4

    Plasmid

    #127652

    Purpose

    Knock-out of human NONO

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

    Insert

    NONO sgRNA (NONO Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pORANGE GFP-CACNA1E KI

    Plasmid

    #131481

    Purpose

    Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacna1e, Cacna1e Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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