We narrowed to 58,607 results for: plasmid
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Plasmid#154769Purposeplasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianMutationY125APromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_mScarlet_CLASP_RGS2membrane
Plasmid#133086PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to blDepositorInsertmScarlet-CLASP
ExpressionBacterial and YeastAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP
Plasmid#159086PurposeExpresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.DepositorInsertempty crRNA-acRNA backbone, dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsEGFP and FLAGExpressionMammalianPromoterhSYN, U6Available SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GRET
Plasmid#158222PurposeControl lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.DepositorInsertGFP-Nluc
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV Retrograde)
Viral Prep#83897-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomatoAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP076
Plasmid#158794PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-1
ExpressionBacterialAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
Plasmid#131461PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53DepositorInsertH3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
UseCre/Lox and Synthetic Biology; Mosaic analysis fo…TagsTrp53-V5, H3F3A-EGFP-AU1ExpressionMammalianMutationPdgfra D842VPromoternone (4x polyA to mitigate episomal expression)Available SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_3xflag_NRF2(RR)
Plasmid#136535PurposeLentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)DepositorInsertNRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianMutationThis plasmid is developed by mutating 3 base pair…Available SinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV Retrograde)
Viral Prep#83896-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomato (physically separate, not a fusion protein)Available SinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
epi-BE4max
Plasmid#135975PurposeExpresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication originDepositorTypeEmpty backboneExpressionMammalianAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL3005
Plasmid#139977PurposeSuicide plasmid for integration of PlacUV5-cscB and lacI into Synechococcus sp. UTEX 2973 Neutral site IIIDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)
Plasmid#139993PurposeCMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationxCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.E222
Plasmid#138248PurposeThree input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promotersDepositorInsertsaraC (araC )
ECF20.N.(VDA).M86.N2
Terminator
SspGyrB.C2.(SAK).ECF16.C
Terminator
rhaS
ECF17.N.(NPC).NrdJ-1.N2
Terminator
M86.C2.(SDL).ECF20.C
Terminator
luxR
Terminator
ECF16.N.(AGG).SspGyrB.N2
Terminator
NrdJ-1.C2.(SEI).ECF17.C
UseSynthetic BiologyExpressionBacterialPromoterJ23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-MYO5C
Plasmid#135404PurposeExpresses GFP-tagged human MYO5C in mammalian cells.DepositorInsertMYO5C (MYO5C Human)
TagsEGFPExpressionMammalianMutationRelative to the GenBank MYO5C cDNA sequence repor…PromoterCMVAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)1
Plasmid#127089PurposeBacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.DepositorAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENO1-iRFP-NATr
Plasmid#129731PurposeIntegration plasmid to overexpress iRFP in Candida albicansDepositorInsertiRFP-Candida albicans codon-optimized
ExpressionYeastPromoterENO1 from Candida albicansAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-eGFP
Plasmid#126717Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
ExpressionYeastPromoterGAPAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCggRO-reporter
Plasmid#124582PurposeReporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)DepositorInsertCggR promoter element
ExpressionYeastAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
BE3-P2A-EGFP (pJUL977)
Plasmid#123612PurposeCAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.DepositorInsertBE3-P2A-EGFP
ExpressionMammalianMutationD10A in SpCas9PromoterCAGAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP/mCherry FLIM/FRET Kit
Plasmid Kit#1000000148PurposePlasmids for analysis of protein-protein interactions in planta via Förster Resonance Energy Transfer (FRET) using EGFP and mCherry.DepositorApplicationCloning and Synthetic Biology, Gene Expression and LabelingVector TypePlant ExpressionCloning TypeGatewayAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only