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pET28a(+)-SUMO-A'

Plasmid

#209115

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-Cre

Plasmid

#167939

Purpose

human CD81-TEV cleavage site-Cre

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD81 (CD81 Human)

Tags

Cre recombinase and TEV protease cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hArfaptin2

Plasmid

#206405

Purpose

Mammalian expression of hArfaptin2

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin2 (ARFIP2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pROSA26-promoter

Plasmid

#21710

Depositor

Philippe Soriano

Article

Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

Insert

ROSA26 promoter (Gt(ROSA)26Sor Mouse)

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pRDB_156

Plasmid

#216090

Purpose

Cas9 [Sp] CRISPRa targeting CD274, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD274 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-Sec61β

Plasmid

#186574

Purpose

Expression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cells

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

SEC61B (SEC61B Human)

Tags

FRB-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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LL - hOCT4i -1

Plasmid

#12198

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Nkx2.2

Plasmid

#64840

Purpose

A lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Nkx2.2 (Nkx2-2 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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ECFP-GalT

Plasmid

#11937

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pMRS-AraC-GFP

Plasmid

#220199

Purpose

mini-Tn7 GFP expression vector - AraC/pBAD promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

AraC/pBAD

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-XylS-GFP

Plasmid

#220200

Purpose

mini-Tn7 GFP expression vector - XylS/pM promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

XylS/pM

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGASΔN-HA

Plasmid

#130912

Purpose

Expresses human cGASΔN (aa160-522)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGASΔN

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-CD4-FLAG

Plasmid

#51604

Purpose

expresses CD4 with C-terminal FLAG tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

CD4 (CD4 Human)

Tags

FLAG and myc

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pRN3P-HA-Suv4-20h2

Plasmid

#86691

Purpose

For transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)

Tags

HA

Promoter

T3

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry2-mito

Plasmid

#164164

Purpose

Expresses mCherry2-fused with a mitochondria-targeting signal of the ActA gene of Listeria monocytogenes under the control of CAG promoter

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2-mtActA

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pME-p3E-HyPer7

Plasmid

#194289

Purpose

Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

Hyper7

Use

Multisite gateway entry clone

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-INPP5E

Plasmid

#171948

Purpose

Expresses INPP5E in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

INPP5E (INPP5E Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-PML-IV-mSIM-C3

Plasmid

#134529

Purpose

Expresses Cherry-PML-IV fusion protein with a mutation in its SIM

Depositor

Susan Janicki

Article

Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

Insert

PML-IV-mSIM

Tags

YFP

Expression

Mammalian

Mutation

SIM (VVVI) 556-559 aa changed to VGGG

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Cep83

Plasmid

#128874

Purpose

For mammalian expression of mouse Cep83

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Cep83 (Ccdc41 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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DYSF-Venus

Plasmid

#29768

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF-Venus (DYSF Human)

Tags

Venus

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only