We narrowed to 10,496 results for: HAE

Showing: 9481 - 9500 of 10496 results

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mEos3.2trLATo

Plasmid

#203746

Purpose

Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLATo (Lat Mouse)

Tags

ER translocation signal - mEos3.2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI

Plasmid

#202596

Purpose

plasmid for expressing the DNMT3bCI (catalytic inactive)

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

H2B-mCherry-T2A-rTetR-DNMT3bCI (Dnmt3b Synthetic, Mouse)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Mutation

P656V, C657D

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_tKiMBImut-T2A-caMEK

Plasmid

#199579

Purpose

Express tKiMBImut(AA) and caMEK in an AAV vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator (mutant)
constitutively active MEK

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13(Y118D)

Plasmid

#188638

Purpose

For expression of human ATG13(Y118D)

Depositor

Michael Lazarou

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Changed tyrosine 118 to aspartic acid

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13(W50D)

Plasmid

#188639

Purpose

For expression of human ATG13(W50D)

Depositor

Michael Lazarou

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Changed tryptophan 50 to aspartic acid

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP-MOORmut

Plasmid

#198213

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_MOORmut

Tags

6x His Tag and mutated (non-permissible) MOORmut …

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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SNAP-Kindlin

Plasmid

#186136

Purpose

For expression of recombinant Snap-labeled kindlin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

FERMT2 (FERMT2 Human)

Tags

His6-sumo

Expression

Bacterial

Promoter

T7

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-gltA1-gltA2

Plasmid

#71348

Purpose

gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

gltA1-gltA2 guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-RanGAP*

Plasmid

#175237

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-E2

Plasmid

#175243

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-RanGAP

Plasmid

#175236

Purpose

Bacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin-FLN-ELP-His-ybbr

Plasmid

#168039

Purpose

Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG-FLN-ELP-His-ybbr

Plasmid

#168047

Purpose

Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G

Tags

3xELP linker, 6x Histag, ddFLN4, and ybbr tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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UAS-u(XXL)Cas9

Plasmid

#127387

Purpose

Expresses Cas9 at very low level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(XXL)-Cas9

Use

CRISPR

Expression

Insect

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

Plasmid

#133348

Purpose

expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_blaA and sgRNA_cpaA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-NBR1(F562A/Y731A/ΔUBD)

Plasmid

#119692

Purpose

For mammalian expression of LIR double mutant NBR1 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

NBR1 (NBR1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

F562A/Y731A & UBD deletion (1-906 ), See depo…

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN iRFP670-OPTN(F178A/D474N)

Plasmid

#119694

Purpose

For mammalian expression of iRFP-tagged LIR/UBD double mutant OPTN

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

OPTN (OPTN Human)

Use

Retroviral

Tags

iRFP670

Expression

Mammalian

Mutation

F178A/D474N

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTG-mTDGa.0

Plasmid

#81048

Purpose

bacterial expression of murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST-Myc-mSesn2 (1186)

Plasmid

#66920

Purpose

expression of mouse Sesn2 in drosophila

Depositor

Michael Karin, Jun Hee Lee

Article

Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

Insert

Sesn2 (Sesn2 Mouse)

Tags

Myc

Expression

Insect

Promoter

HS promoter

Available Since

Dec. 26, 2017

Availability

Academic Institutions and Nonprofits only

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mIFP-C-Src-7

Plasmid

#56213

Purpose

Localization: Membrane/Vesicles, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

C-Src (SRC Chicken)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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