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pLPT1

Plasmid

#209995

Purpose

Contains Level 0 Part: Terminator (TB1006) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

Terminator (TB1006)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPS1

Plasmid

#209992

Purpose

Contains Level 0 Part: Ribosome binding site (RB0034) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

RBS (RB0034)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-WDR5-P2A-SH2-P2A-Ras-CAAX

Plasmid

#209712

Purpose

Mammailan expression of WDR5, SH2, and HRas separated by P2A.

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

WDR5-P2A-SH2-P2A-Ras-CAAX

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-NLS-MBP-rATOM(WDR5)

Plasmid

#209710

Purpose

Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

NLS-MBP-rATOM(WDR5)

Tags

NLS-MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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GreenT-EC.b - pRSET

Plasmid

#193948

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.b

Tags

His-Tag

Expression

Bacterial

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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GreenT-EC.c - pRSET

Plasmid

#193949

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.c

Tags

His-Tag

Expression

Bacterial

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT255-ameRS

Plasmid

#202309

Purpose

ameRS repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

ameRS

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT252-amtR

Plasmid

#202307

Purpose

amtR repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

amtR

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT251-bm3R1

Plasmid

#202306

Purpose

bm3R1 repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

bm3R1

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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plYJP067-PcymRC_v2

Plasmid

#202302

Purpose

PCymRC_v2-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

PCymRC_v2-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT243-Pcin

Plasmid

#202301

Purpose

PCin-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

Pcin-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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plYJP067-Pttg

Plasmid

#202300

Purpose

PTtg-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

PTtg-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT238-Pbad

Plasmid

#202298

Purpose

PBad-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

Pbad-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT237-Ptac

Plasmid

#202297

Purpose

PTac-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

PTac-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT475

Plasmid

#202292

Purpose

RPU plasmid w/ high RBS (p15a backbone)

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

J23101-RiboJ00-B0034-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb713-P2A-GFP

Plasmid

#197172

Purpose

Expresses the protein of mRhubarb713-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubard713-P2A-GFP

Use

AAV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP2-P2A-GFP

Plasmid

#197155

Purpose

Expresses the protein of miRFP2-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP2-P2A-GFP

Use

AAV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 AMSHLP 16-218

Plasmid

#180609

Purpose

Bacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

AMSHLP residues 16-218

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 16-218

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-BpBBS

Plasmid

#199468

Purpose

Expression of the biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Manoilov et al J Mol Biol. 2022 Jan 30;434(2):167359. d

Insert

BpBBS

Expression

Mammalian

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-XRCC6 zeo

Plasmid

#192327

Purpose

Lentiviral expression vector for an inducible 3xFLAG-XRCC6

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

XRCC6 (XRCC6 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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