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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,205 results for: LEA

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Showing: 9501 - 9520 of 12205 results

  5. pToxEXPr6

    Plasmid
    #218290
    Purpose
    Introduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3
    Depositor
    Claudia Vickers
    Publication
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    hph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
    Expression
    Yeast
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218290-plasmid-map-sequence-id-433735

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  6. pCL1314

    Plasmid
    #217956
    Purpose
    Expression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Publication
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    GGG insertion between A95 and C96
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217956-plasmid-map-sequence-id-430210

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  11. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid
    #202588
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Publication
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, changed O…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202588-plasmid-map-sequence-id-415632

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  16. pRSF-G1TMSNKRS

    Plasmid
    #198323
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Publication
    Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac
    Insert
    tRNA synthetase
    Expression
    Bacterial
    Promoter
    T7
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    198323-plasmid-map-sequence-id-393448

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  17. pET15b I-SceI (Ctermhis) K122I

    Plasmid
    #195614
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Publication
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Expression
    Bacterial
    Mutation
    C2458G, A2826T, A2827T
    Promoter
    T7
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195614-plasmid-map-sequence-id-383676

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Showing: 9501 - 9520 of 12205 results