We narrowed to 35,282 results for: puro

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pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236362

Purpose

Overexpression of HyperdAsCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdAsCas12a

Use

CRISPR and Lentiviral

Mutation

E174R, D256R, E321R, E371R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236361

Purpose

Overexpression of dAsCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dAsCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)

Plasmid

#238181

Purpose

Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF5 (KLF5 Human, Synthetic)

Use

Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)

Plasmid

#238184

Purpose

Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF5 (KLF5 Human, Synthetic)

Use

Lentiviral

Tags

mTagBFP2

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

Plasmid

#232244

Purpose

Mammalian expression of N581K RLIM-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

N581K RLIM-GFP-IRES-mCherry (RLIM Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

N581K

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro

Plasmid

#212675

Purpose

Express tagged hRb phosphosite mutant

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

Rb (RB1 Human)

Use

Lentiviral

Tags

HA

Mutation

15 CDK phospho-sites are mutated to alanines, and…

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

Plasmid

#221385

Purpose

Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

Depositor

Junwei Shi

Article

Ngiow et al Cell. 2024 Aug 8;187(16):4336-4354.e19.

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-L17-Q2L7-T2176C-UGI-Puro

Plasmid

#209646

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

L17-Q2L7-T2176C

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-R16-Q2L7-T2176N-UGI-Puro

Plasmid

#209649

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

R16-Q2L7-T2176N

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-R16-FZY2-S100N-UGI-Puro

Plasmid

#209644

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

R16-FZY2-S100N

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-R16-FZY2-S100C-UGI-Puro

Plasmid

#209643

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

R16-FZY2-S100C

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR

Plasmid

#171965

Purpose

Donor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

Insert

Synthetic CLTX CD32a ITAM CD3z CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124286

Purpose

sgRNA against human Col7A1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Collagen type VII alpha 1 chain (COL7A1 Human)

Use

CRISPR

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124288

Purpose

sgRNA against human NFATc3

Depositor

Katalin Josvay, Csaba Vizler

Insert

NFATc3 nuclear factor of activated T cells (NFATC3 Human)

Use

CRISPR

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

Plasmid

#120565

Purpose

Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K, T35A

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

Plasmid

#120567

Purpose

Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K, Y40A

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

Plasmid

#120566

Purpose

Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K, E37A

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set B

Pooled Library

#92386

Purpose

Crispri

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Use

Lentiviral

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only
pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

Plasmid

#218557

Purpose

Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-HSF1-VIRF2

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)

Plasmid

#214878

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF1 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF1-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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