We narrowed to 5,282 results for: pcdna3.1

1. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

   Plasmid

   #159132

   Purpose

   Expresses BACH1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   BACH1 (BACH1 Human)
   

   Use

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Mutation

   Y11F

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-TTF-1-HDD

   Plasmid

   #52063

   Purpose

   It encodes a mutant of TTF-1 in which the homeodomain is deleted

   Depositor

   David Mu

   Article

   Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

   Insert

   Thyroid Transcription Factor 1 (NKX2-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Homeodomain deleted

   Promoter

   Available since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1-CHRFAM7A-mGFP

   Plasmid

   #62633

   Purpose

   mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loop

   Depositor

   Henry Lester

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   CHRFAM7A (CHRFAM7A Human)
   

   Use

   Tags

   mEGFP inserted in M3-M4 loop

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1+_LN-HA-AGO2-WT

   Plasmid

   #92009

   Purpose

   Expresses Lambda-N-HA-AGO2 (WT)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   Lambda-N-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY117 (pcDNA3.1-huMb3Cpf1)

   Plasmid

   #92293

   Purpose

   Expression of humanized Mb3Cpf1

   Depositor

   Feng Zhang

   Insert

   huMb3Cpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 MYC dLZ

   Plasmid

   #176048

   Purpose

   Expresses Myc Mutant (delta leucine zipper) in Mammalian cells

   Depositor

   Linda Penn

   Article

   Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

   Insert

   Myc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted amino acids 414-433

   Promoter

   CMV

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-Rab10-FLAG

   Plasmid

   #135559

   Purpose

   Express mouse Rab10 in mammalian cells, FLAG at N terminal

   Depositor

   Jingshi Shen

   Article

   Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   Rab10 (Rab10 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   T7 promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-FluoSTEP-AKAR

   Plasmid

   #181844

   Purpose

   Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   FluoSTEP-AKAR
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-alpha4 wt (Hygro)

   Plasmid

   #80017

   Purpose

   Mammalian expression of human integrin alpha4, Hygro selection

   Depositor

   Chinten James Lim

   Article

   Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

   Insert

   integrin alpha4 (ITGA4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pY120 (pcDNA3.1-huFbCpf1)

    Plasmid

    #92296

    Purpose

    Expression of humanized FbCpf1

    Depositor

    Feng Zhang

    Insert

    huFbCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Rat NCX1.3 (pcDNA3.1+)

    Plasmid

    #75191

    Purpose

    Mammalian expression of SLC8A1

    Depositor

    Jonathan Lytton

    Article

    Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

    Insert

    SLC8A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-MS2-HA-HsNeuroguidin_AA

    Plasmid

    #148237

    Purpose

    Mammalian Expression of HsNeuroguidin

    Depositor

    Elisa Izaurralde

    Insert

    HsNeuroguidin (NGDN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Hyg-BFP

    Plasmid

    #214363

    Purpose

    Expression of the Blue Fluorescent Protein

    Depositor

    Bertrand Collet

    Article

    Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

    Insert

    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 FLIPE-600n

    Plasmid

    #13541

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E WT
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-6His-MyoX

    Plasmid

    #47607

    Depositor

    Emanuel Strehler

    Article

    Bennett et al Biochem Biophys Res Commun. 2008 May 2;3

    Insert

    MyoX (MYO10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q680R

    Promoter

    CMV

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1_mGFP-FGFR3-G380R

    Plasmid

    #191755

    Purpose

    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    c.1138G>A (G380R) + c.1131C>T (silent mutat…

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 FLIPglu-170nDelta13V

    Plasmid

    #18018

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu F16F Delta13
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    WT binding protein (no F16A)

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)_Paragranulin+linker 1

    Plasmid

    #176915

    Purpose

    express Paragranulin with linker 1 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Paragranulin + linker 1 (GRN Human)
    

    Use

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_mGFP-FGFR3-mScarlet

    Plasmid

    #192509

    Purpose

    Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc-mScarlet
    

    Use

    Tags

    mGFP and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1+ Flag-RAGB99L

    Plasmid

    #128101

    Purpose

    Expresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmid

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    Ras related GTP binding B (RRAGB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q99L (CAA to CTA)

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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