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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 36,026 results for: CaS

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Showing: 9621 - 9640 of 36026 results

  1. pPHB.1.0-gDNA

    Plasmid
    #132453
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    PHB
    Use
    CRISPR
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    132453-plasmid-map-sequence-id-266831

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  2. C113m

    Plasmid
    #121012
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Cas9
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121012-plasmid-map-sequence-id-236320

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  3. pFG001

    Plasmid
    #62816
    Purpose
    Bacterial constitutive gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PN25
    Available Since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    62816-plasmid-map-sequence-id-123824

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  6. pCMV-eVRQR-P2A-EGFP (KAC1028)

    Plasmid
    #242663
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    eVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242663-plasmid-map-sequence-id-489032

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  8. pXPR_016

    Plasmid
    #107143
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    David Root
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available Since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    107143-plasmid-map-sequence-id-205293

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  10. pHnS 2comp KI;Empty

    Plasmid
    #240302
    Purpose
    Empty vector
    Depositor
    Yuki Ogawa
    Insert
    Empty gRNA
    Use
    AAV
    Mutation
    NA
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240302-plasmid-map-sequence-id-478528

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  11. pAAV 3xgRNA KO;Template

    Plasmid
    #240310
    Purpose
    KO:Template
    Depositor
    Yuki Ogawa
    Insert
    Template gRNA
    Use
    AAV
    Mutation
    NA
    Available Since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240310-plasmid-map-sequence-id-478217

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  12. pPB-CAG-PlatTET-wpA-pPuroTK

    Plasmid
    #214125
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    dCas9-TET
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214125-plasmid-map-sequence-id-424237

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  13. lentiGuide_puro-P2A-mNeonGreen

    Plasmid
    #221464
    Purpose
    Lentiviral expression of an sgRNA with mNeonGreen fluorescent marker
    Depositor
    James Eshleman
    Insert
    mNeonGreen
    Use
    CRISPR and Lentiviral
    Promoter
    hU6, EF-1a
    Available Since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    221464-plasmid-map-sequence-id-438191

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  14. pBHM2651

    Plasmid
    #229993
    Purpose
    Carries AID* tag, empty sgRNA, and NEOR marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - NEOR
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229993-plasmid-map-sequence-id-461132

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  15. pY130 (pcDNA3.1-U6-huBsCpf1)

    Plasmid
    #92306
    Purpose
    Expression of humanized BsCpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huBsCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92306-plasmid-map-sequence-id-181649

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  16. pY128 (pcDNA3.1-U6-huMb2Cpf1)

    Plasmid
    #92304
    Purpose
    Expression of humanized Mb2Cpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huMb2Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92304-plasmid-map-sequence-id-181652

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  17. pY129 (pcDNA3.1-U6-huMb3Cpf1)

    Plasmid
    #92305
    Purpose
    Expression of humanized Mb3Cpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huMb3Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92305-plasmid-map-sequence-id-181650

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  18. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid
    #92303
    Purpose
    Expression of humanized TsCpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huTsCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92303-plasmid-map-sequence-id-181636

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  19. 4MT-D3-CPV

    Plasmid
    #58184
    Purpose
    Localization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , Emission: 475 / 528
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    ECFP and Venus
    Expression
    Mammalian
    Available Since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pRR2

    Plasmid
    #166097
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166097-plasmid-map-sequence-id-329109

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Showing: 9621 - 9640 of 36026 results