We narrowed to 16,198 results for: grna

1. Cdk13_intron4_sg3_pX330

   Plasmid

   #127340

   Purpose

   sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #3

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Mouse Cdk13 Intron 4 Targeting sgRNA (Cdk13 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6 Promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CIRTS-YTHDF2-Malat1

   Plasmid

   #216858

   Purpose

   CIRTS RNA targeting system for targeted knockdown of m6A-containing Malat1 at the synapse. CIRTS-YTHDF2-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.

   Depositor

   Timothy Bredy

   Article

   Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

   Insert

   CIRTS-YTHDF2-Calm3-U6-Malat1 gRNA
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Syn1, U6

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-KrasG13S/sgKras/Cre

   Plasmid

   #99859

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-KrasG13A/sgKras/Cre

   Plasmid

   #99855

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-KrasG13R/sgKras/Cre

   Plasmid

   #99858

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-tTRKRAB-red

   Plasmid

   #12250

   Depositor

   Didier Trono

   Article

   Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

   Insert

   TetR-KRAB fusion and dsRED
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Mutation

   dsRed added to backbone.

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-blast shLuc

   Plasmid

   #110409

   Purpose

   shLuc (Target TTACGCTGAGTACTTCGA), silence LUC gene, blasticidin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   Luciferase
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 (RNA PolIII)

   Available Since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBFv-U6.2

   Plasmid

   #138400

   Purpose

   gRNA expression vector for Drosophila

   Depositor

   Shu Kondo

   Article

   Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   U6-2

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. hCtRNA_FT

   Plasmid

   #186715

   Purpose

   Fragment template for DAP array

   Depositor

   Xue Gao

   Article

   Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

   Insert

   gRNA scaffold and hCtRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hCtRNA

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hCtRNA_VT

    Plasmid

    #186716

    Purpose

    Vector template for DAP array

    Depositor

    Xue Gao

    Article

    Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

    Insert

    hCtRNA and gRNA scaffold
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hCtRNA

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TMEM165 g2 LentiCRISPRv2-mCherry

    Plasmid

    #218661

    Purpose

    Knockout vector for human TMEM165

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    TMEM165 (TMEM165 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3

    Plasmid

    #159911

    Purpose

    Mutagenesis of Kcnq3

    Depositor

    Larry Zweifel

    Insert

    Kcnq3 (Kcnq3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. shGFP neo

    Plasmid

    #72571

    Purpose

    Hairpin targeting GFP.

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSICO-Flpo

    Plasmid

    #24969

    Depositor

    Tyler Jacks

    Article

    Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 10

    Insert

    Flp recombinase
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6-shRNA pgkCre

    Plasmid

    #24971

    Depositor

    Tyler Jacks

    Article

    Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 10

    Insert

    shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO-Tet-puro-hRAF1-shRNA-1

    Plasmid

    #185371

    Purpose

    For tetracycline-inducible mammalian expression of shRNA: CATGAGTATTTAGAGGAAGTA that targets human RAF1

    Depositor

    Ayaz Najafov

    Insert

    RAF1 (RAF1 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVTH-siGFP

    Plasmid

    #12248

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Insert

    shRNA against GFP
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Mutation

    NGFRdel is in place of GFP on the backbone.

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. MDH1-miR-223-PGK-GFP

    Plasmid

    #11378

    Depositor

    Chang-Zheng Chen

    Article

    Chen et al Science. 2004 Jan 2. 303(5654):83-6.

    Insert

    miR-223
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pV1081

    Plasmid

    #111425

    Purpose

    Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgADE-NT2
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. MDH1-miR-142-PGK-GFP

    Plasmid

    #11377

    Depositor

    Chang-Zheng Chen

    Article

    Chen et al Science. 2004 Jan 2. 303(5654):83-6.

    Insert

    miR-142
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only