We narrowed to 166,321 results for: addgene

1. pDK324

   Plasmid

   #134190

   Purpose

   Expression of C. elegans KNL1 with 8A mutation

   Depositor

   Iain Cheeseman

   Insert

   KNL1 (knl-1 Nematode)
   

   Expression

   Bacterial

   Mutation

   8A

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDAX-EMMI-034 EB3(D-G)//Esp3I

   Plasmid

   #130236

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   EB3
   

   Use

   Synthetic Biology

   Available Since

   Feb. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGGD_RT_biChromium

   Plasmid

   #136974

   Purpose

   Goldengate/Greengate C-tag module, containing a dual fluorescent marker based on the P2A peptide

   Depositor

   Klaus Theres

   Insert

   P2A sequence
   

   Expression

   Bacterial

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGI_RT_Sa_sgRNA

   Plasmid

   #136982

   Purpose

   Goldengate/Greengate module for genome editing, using the Sa guideRNA.

   Depositor

   Klaus Theres

   Insert

   Sa guide RNA
   

   Use

   CRISPR

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGGD_RT_Sa_sgRNA

   Plasmid

   #136975

   Purpose

   Goldengate/Greengate module for genome editing, using the Sa guideRNA.

   Depositor

   Klaus Theres

   Insert

   Sa guide RNA
   

   Use

   CRISPR

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGD_RT_li_Tq2CFP_2NLS

   Plasmid

   #136972

   Purpose

   Goldengate/Greengate C-tag module, containing Tq2CFP fused to 2 SV40 NLS and a flexible linker

   Depositor

   Klaus Theres

   Insert

   Tq2CFP
   

   Expression

   Bacterial

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGGE_RT_Sa_sgRNA

   Plasmid

   #136978

   Purpose

   Goldengate/Greengate module for genome editing, using the Sa guideRNA.

   Depositor

   Klaus Theres

   Insert

   Sa guide RNA
   

   Use

   CRISPR

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. C77m

   Plasmid

   #120989

   Purpose

   MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   AmpR
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. C78m

   Plasmid

   #120990

   Purpose

   MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   ChlorR
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pExp-DsbC(SS)

    Plasmid

    #129243

    Purpose

    Protein expression in E.coli

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    His and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. U6-Mos-as

    Plasmid

    #120534

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Mos (Mos Mouse)
    

    Use

    RNAi

    Mutation

    A>G (see depositor comments)

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. H1-Mos-6U

    Plasmid

    #120531

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Mos (Mos Mouse)
    

    Use

    RNAi

    Mutation

    A>G (see comments)

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AtTPT (ORF-HIS)

    Plasmid

    #117092

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    AtTPT (ORF-HIS)
    

    Tags

    His

    Expression

    Yeast

    Promoter

    AOX

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCWX-R4DESTR2-PGK-Tk:Sh

    Plasmid

    #114321

    Purpose

    Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Zeocin/Bleomycin resistance and Acyclovir conditional ablation

    Depositor

    Patrick Salmon

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOP5TT

    Plasmid

    #112613

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8His-thioredoxin and Avi

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOP5BP

    Plasmid

    #112608

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8His-GB1 and Avi

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pORFE1001

    Plasmid

    #112079

    Purpose

    Overexpression of the AtCAS9 in plant under 2x35S promoter

    Depositor

    Christian Chevalier

    Insert

    AtCAS9
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Plant codon optimized Cas9 S. pyogenes

    Promoter

    2x35S

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS315 U2 IIc+

    Plasmid

    #111357

    Purpose

    U2 snRNA with IIc+ mutation on a pRS315 plasmid

    Depositor

    Christine Guthrie

    Insert

    snR20
    

    Expression

    Yeast

    Mutation

    stem II IIc+ mutation

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pORFE0002

    Plasmid

    #112071

    Purpose

    AtCAS9 nickase D10A module CDS1

    Depositor

    Christian Chevalier

    Insert

    AtCAS9 nickase D10A
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Plant codon optimized nCas9 D10A S. pyogenes

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pORFE0003

    Plasmid

    #112072

    Purpose

    AtDeadCAS9 D10A H840A module CDS1

    Depositor

    Christian Chevalier

    Insert

    AtDeadCAS9 D10A H840A
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Plant codon optimized dCas9 D10A S. pyogenes

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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