205,044 results

1. pCMV-3×FLAG-SLC35A2

   Plasmid

   #186283

   Purpose

   transient expression of N-terminal 3×FLAG tagged SLC35A2 isoform c/UGT1 in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

   Insert

   SLC35A2 (SLC35A2 Human)
   

   Use

   Tags

   3×FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIS.MTRIA-NKB

   Plasmid

   #184609

   Purpose

   For expression of a fluorescent sensor for neurokinin B in mammalian cells

   Depositor

   Hiroshi Hibino, Daisuke Ino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-NKB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIS.MTRIA-NPFF

   Plasmid

   #184612

   Purpose

   For expression of a fluorescent sensor for neuropeptide FF in mammalian cells

   Depositor

   Hiroshi Hibino, Daisuke Ino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-NPFF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_eA3A T31A-NG-nCas9

   Plasmid

   #163539

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-RB1-shRNA63

   Plasmid

   #25641

   Purpose

   3rd generation lentiviral vector

   Depositor

   Todd Waldman

   Article

   Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

   Insert

   RB1-shRNA 63 (RB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Cytb5

   Plasmid

   #182579

   Purpose

   Expresses mCherry targeted to the ER via the CytB5RR tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   mCherry-Cytb5
   

   Use

   Tags

   Cytb5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TagBFP2-CAAX

   Plasmid

   #116856

   Purpose

   plasma membrane marker

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   HRAS
   

   Use

   Tags

   TagBFP2

   Expression

   Mammalian

   Mutation

   Amino acids 172-189

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-Mff

   Plasmid

   #49153

   Purpose

   mammalian expression of Mff fused to GFP

   Depositor

   Gia Voeltz

   Article

   Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

   Insert

   Mff (MFF Human)
   

   Use

   Tags

   acGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

   Plasmid

   #193356

   Purpose

   VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   SOX2-17 is engineered highly cooperative chimeric…

   Promoter

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-AR-LBD

    Plasmid

    #171239

    Purpose

    Mammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEBB HA RelA

    Plasmid

    #74892

    Purpose

    Express RelA in mammalian cells

    Depositor

    Ezra Burstein, Colin Duckett

    Article

    Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

    Insert

    RelA (RELA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK792

    Plasmid

    #8830

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219P mutant
    

    Use

    Tags

    His, MBP (cleaved), and polyarginine

    Expression

    Bacterial

    Mutation

    S219P mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 - HA-KLF4 FL

    Plasmid

    #34593

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX551

    Plasmid

    #60957

    Purpose

    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

    Depositor

    Feng Zhang

    Article

    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

    Insert

    SpCas9
    

    Use

    AAV and CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    pMecp2

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C3-Exo70

    Plasmid

    #53761

    Purpose

    pEGFP-C3 plasmid that expresses Exo70

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Exo70 (EXOC7 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. VPS13A^mCherry

    Plasmid

    #118758

    Purpose

    VPS13A internally tagged with mCherry at residue 1372.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13A (VPS13A Human)
    

    Use

    Tags

    mCherry inserted at position 1372

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDN-Cherry-sgRNA

    Plasmid

    #221387

    Purpose

    episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

    Depositor

    Deborah Hung

    Article

    Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

    Insert

    none, cloning vector for introduction of targeting sequences
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTO135_pENO1-CaCas9-tCyc1

    Plasmid

    #171103

    Purpose

    Expresses Cas9 in Candida auris

    Depositor

    Teresa O'Meara

    Article

    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

    Insert

    CaCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO1

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAK_EF1a_CasRx_Hygro

    Plasmid

    #134840

    Purpose

    Lentiviral expression of CasRx T2A Hygromycin

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR and Lentiviral

    Tags

    T2A Hygromycin

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid

    #36047

    Purpose

    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA(R118G)-HA
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    Available since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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