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CSNK1A1_pLX307

Plasmid

#98326

Purpose

Lentiviral expression of CSNK1A1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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CSNK1A1_pLX307

Plasmid

#98326

Purpose

Lentiviral expression of CSNK1A1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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lenti-UCOE-dCas9-BFP-Zim3-KRAB

Plasmid

#196716

Purpose

CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

Depositor

Bernhard Schmierer

Insert

dCas9-TagBFP-KRAB(ZIM3)

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Mutation

D10A, N863A

Promoter

EF1a

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-UCOE-dCas9-BFP-Zim3-KRAB

Plasmid

#196716

Purpose

CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

Depositor

Bernhard Schmierer

Insert

dCas9-TagBFP-KRAB(ZIM3)

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Mutation

D10A, N863A

Promoter

EF1a

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113344

Purpose

Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VP64-dCas9-p300core-T2A-MS2-p65-HSF1

Use

Piggybac

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113344

Purpose

Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VP64-dCas9-p300core-T2A-MS2-p65-HSF1

Use

Piggybac

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSKB-

Plasmid

#62540

Purpose

Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

Depositor

Eric Marois

Publication

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Bacterial

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSKB-

Plasmid

#62540

Purpose

Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

Depositor

Eric Marois

Publication

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Bacterial

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV_SEPT9_i1-msfGFP

Plasmid

#180328

Purpose

mammalian expression of human SEPT9_i1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Publication

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-msfGFP

Plasmid

#180328

Purpose

mammalian expression of human SEPT9_i1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Publication

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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Strep-KIFC1*-HA

Plasmid

#120171

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag

Depositor

Juan Bonifacino

Publication

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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Strep-KIFC1*-HA

Plasmid

#120171

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag

Depositor

Juan Bonifacino

Publication

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCSN061

Plasmid

#101725

Purpose

Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

Depositor

Rene Verwaal

Publication

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Inserts

S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Promoter

Heterologous TEF1 promoter from A. gossypii. and …

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCSN061

Plasmid

#101725

Purpose

Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

Depositor

Rene Verwaal

Publication

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Inserts

S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Promoter

Heterologous TEF1 promoter from A. gossypii. and …

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEYFP-Sec16A

Plasmid

#36156

Purpose

Expresses Sec16A as a YFP fusion

Depositor

David Stephens

Publication

Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sec16A (SEC16A Human)

Tags

EYFP

Expression

Mammalian

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-Sec16A

Plasmid

#36156

Purpose

Expresses Sec16A as a YFP fusion

Depositor

David Stephens

Publication

Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sec16A (SEC16A Human)

Tags

EYFP

Expression

Mammalian

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pBS mPax2 (CT#670)

Plasmid

#13960

Depositor

Cliff Tabin

Publication

Heanue et al Mech Dev. 2002 Feb . 111(1-2):75-87.

Insert

Pax2 (Pax2 Mouse)

Expression

Bacterial

Mutation

This is not full lenght Pax2. There is a 100 nunc…

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBS mPax2 (CT#670)

Plasmid

#13960

Depositor

Cliff Tabin

Publication

Heanue et al Mech Dev. 2002 Feb . 111(1-2):75-87.

Insert

Pax2 (Pax2 Mouse)

Expression

Bacterial

Mutation

This is not full lenght Pax2. There is a 100 nunc…

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry

Plasmid

#122957

Purpose

Lentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expression

Depositor

Pierre Vanderhaeghen

Publication

Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

Insert

NOTCH2NLB (NOTCH2NLB Human)

Use

Lentiviral

Tags

6xmyc tag

Promoter

CAG promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry

Plasmid

#122957

Purpose

Lentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expression

Depositor

Pierre Vanderhaeghen

Publication

Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

Insert

NOTCH2NLB (NOTCH2NLB Human)

Use

Lentiviral

Tags

6xmyc tag

Promoter

CAG promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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