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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,083 results for: pET

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Showing: 9661 - 9680 of 10083 results

  3. pDonorRMCE-Rev-FRT3-MCS-mTagBFP2wintron-noSV40pA-FRT

    Plasmid
    #253426
    Purpose
    Donor plasmid for FLP/FRT mediated recombination into landing pad in the reverse direction with MCS for promoter integrations, without an SV40 polyA signal after reporter
    Depositor
    Joanna Wysocka
    Publication
    Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41
    Type
    Empty backbone
    Use
    Mouse Targeting; Flp/frt
    Tags
    mTagBFP2 with intron
    Expression
    Mammalian
    Available since
    July 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    253426-plasmid-map-sequence-id-504495

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  8. pGTM-Sumo2-WN-NS2_3_TEE

    Plasmid
    #254047
    Purpose
    Expresses West Nile Virus NS3 and an NS2 fragment on a polyistronic message for E. coli expression
    Depositor
    Gaetano Montelione
    Inserts
    NS3
    NS2
    Tags
    6xHis, Sumo
    Expression
    Bacterial
    Promoter
    Expressed as the second ORF in a polycistronic me…
    Available since
    May 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    254047-plasmid-map-sequence-id-509978

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  9. pGTM-Sumo2-Zika-NS2-NS3

    Plasmid
    #240390
    Purpose
    Expresses Zika virus protease in E. coli
    Depositor
    Gaetano Montelione
    Insert
    NS2B-NS3 protease
    Tags
    6xHis, Sumo
    Expression
    Bacterial
    Mutation
    NS2B-NS3 peptides fused by Gly-Ser linker
    Promoter
    T7 lac
    Available since
    May 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    240390-plasmid-map-sequence-id-505754

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  10. pGTM_Sumo2-N-StrepII-PLpro

    Plasmid
    #234322
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease Cleavage a Strep-tag II sequence is retained at the N-terminus
    Depositor
    Gaetano Montelione
    Publication
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and Strep-tag II
    Expression
    Bacterial
    Promoter
    T7 lac
    Available since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234322-plasmid-map-sequence-id-469910

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  11. pGTM_Sumo2-N-8XHis-Plpro-C111S

    Plasmid
    #234472
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 C111S with an activity abolishing mutation for the purification of this PLpro protease. Following Sumo Protease Cleavage an N-term 8xHis Tag is retained
    Depositor
    Gaetano Montelione
    Publication
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and 8xHis
    Expression
    Bacterial
    Promoter
    T7 lac
    Available since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234472-plasmid-map-sequence-id-469247

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  12. M24

    Plasmid
    #242619
    Purpose
    Strep- MYC deltaCTD, deltaMB0-1-His protein expression in bacteria
    Depositor
    Alain Nepveu
    Publication
    Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    Strep- MYC deltaCTD, deltaMB0-1-His (MYC Human)
    Tags
    His and Strep
    Expression
    Bacterial
    Mutation
    delta2-62, delta354-439
    Available since
    Sept. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    242619-plasmid-map-sequence-id-486690

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Showing: 9661 - 9680 of 10083 results