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pAG415Gal_Gcn4 ILVtoED WGFP

Plasmid

#233007

Purpose

Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoAS

Plasmid

#232959

Purpose

Galactose iduced expression of Gcn4 ILVtoAS in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol W

Plasmid

#231878

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol

Plasmid

#231877

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-APOBEC1-EGFP

Plasmid

#209324

Purpose

AAV packaging vector containing a APOBEC1 expression, a P2A-EGFP expression cassette.

Depositor

Magdalena Koziol

Article

Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

Insert

APOBEC1-HA

Use

AAV

Tags

HA

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-K435L-L438K-peGFP-N1

Plasmid

#228587

Purpose

Expresses OSCA1.2-K435L-L438K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Expression

Mammalian

Mutation

K435L, L438K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a Promoter (MTK 2) (pAN2824)

Plasmid

#194225

Purpose

Mammalian Toolkit part 2 containing the EF1a promoter

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

EF1a Promoter

Use

Synthetic Biology

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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LCB1_v1.3 (MTK 3a) (pZYW044)

Plasmid

#194227

Purpose

Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB1

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

LCB1_v1.3

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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LCB3_v1.2 (MTK 3a) (pZYW045)

Plasmid

#194228

Purpose

Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB3

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

LCB3_v1.2

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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WPRE (MTK 4b) (pAN2410)

Plasmid

#194235

Purpose

Mammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbones

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

WPRE terminator

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuideSLC35C1-neo

Plasmid

#220868

Purpose

encodes CRISPR sgRNA targeting human SLC3C1

Depositor

Jennifer Kohler

Article

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

SLC35C1 (SLC35C1 Human)

Use

Lentiviral

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MOV10-ts3

Plasmid

#174279

Purpose

MOV10 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MOV10 shRNA (MOV10 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1312-AAV-EFSNC-dSaCas9-KRAB

Plasmid

#223157

Purpose

Expression of KRAB with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-eGFP-NES-NFATC2IP

Plasmid

#221446

Purpose

Mammalian expression vector of eGFP tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminus

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

Nuclear Export Signal (NES) and eGFP

Expression

Mammalian

Mutation

NES (NLVDLQKKLEELELDEQQ) inserted, 1st Methionine…

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-3xFLAG-NFATC2IP ∆101-140

Plasmid

#221212

Purpose

Mammalian expression vector of triple Flag tagged NFATC2IP with amino acids 101-140 deleted

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

3XFlag

Expression

Mammalian

Mutation

1st Methionine and amino acids 101-140 deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-3XFLAG-NFATC2IP SLD2

Plasmid

#221388

Purpose

Mammalian expression vector of triple Flag tagged NFATC2IP ORF SUMO-like domain 2 (SLD2, aa 345-418)

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Amino acids 1-344 of NFATC2IP deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-3XFLAG-SLD1+SLD2

Plasmid

#221389

Purpose

Mammalian expression vector of triple Flag tagged NFATC2IP ORF SUMO-like domain 1 and SUMO-like domain 2 (SLD1+SLD2, aa 251-419)

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Amino acids 1-250 of NFATC2IP deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-eGFP-NFAT2CIP ∆101-140

Plasmid

#221437

Purpose

Mammalian expression vector of eGFP tagged NFATC2IP with amino acids 101-140 deleted

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

eGFP

Expression

Mammalian

Mutation

1st Methionine and amino acids 101-140 deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-eGFP-NFATC2IP SLD2

Plasmid

#221438

Purpose

Mammalian expression vector of eGFP tagged NFATC2IP SUMO-like domain 2 (SLD2, aa 345-418)

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

eGFP

Expression

Mammalian

Mutation

Amino acids 1-344 of NFATC2IP deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-eGFP-SLD1+SLD2

Plasmid

#221443

Purpose

Mammalian expression vector of egFP tagged NFATC2IP ORF SUMO-like domain 1 and SUMO-like domain 2 (SLD1+SLD2, aa 251-419)

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

eGFP

Expression

Mammalian

Mutation

Amino acids 1-250 of NFATC2IP deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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