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pUPD3_PARTPRM_tetR_Ptet

Plasmid

#243924

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetR and PtetA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_cI857_PR

Plasmid

#243926

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cI857 and PR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PT7

Plasmid

#243928

Purpose

GoldenBraid2.0 T7 RNA polymerase-dependent promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PT7lac

Plasmid

#243929

Purpose

GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7lac

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_lacI_Plac

Plasmid

#243909

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

lacI and PlacZYA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_araC_Para

Plasmid

#243911

Purpose

GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

araC and ParaBAD

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_tetR_Ptet

Plasmid

#243913

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetR and PtetA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD3_FULLPRM_cI857_PR

Plasmid

#243915

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cI857 and PR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PT7

Plasmid

#243917

Purpose

GoldenBraid2.0 T7 RNA polymerase-dependent promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PT7lac

Plasmid

#243918

Purpose

GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7lac

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKo1_Stuffer1_Stuffer2

Plasmid

#243896

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an omega-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer1 and Stuffer2

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKo2_Stuffer2_Stuffer1

Plasmid

#243897

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an omega-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer2 and Stuffer1

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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ptetgRNA-FRT

Plasmid

#243872

Purpose

sgRNA expression vector under tetR transcriptional control.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

sgRNA_FRT

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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POC1551

Plasmid

#226498

Purpose

pMOBK360_StandVKL: Standard Vector (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1552

Plasmid

#226499

Purpose

pMOBC360_StandVC: Standard Vector (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1423

Plasmid

#221559

Purpose

Plasmid designed to assay the site-selective methylation protection approach using the non-switchable methylases

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE mTFP1-actin

Plasmid

#229970

Purpose

encodes a mTFP1-actin fusion construct under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

mTFP1-actin

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE H2B iRFP720

Plasmid

#229968

Purpose

encodes a H2B-iRFP720 fusion contruct under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

H2B iRFP720

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE mito-mCherry

Plasmid

#229966

Purpose

encodes a mCherry targeted to mitochondria under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

mito-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB EF1alpha

Plasmid

#229954

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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