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pCMV-dCas12a-ABE7

Plasmid

#193644

Purpose

Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA-TadA*7.10, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTNwt-FL

Plasmid

#110217

Purpose

Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Expression

Bacterial

Mutation

P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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H3S10ph biosensor

Plasmid

#120809

Purpose

FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP

Expression

Mammalian

Mutation

Threonine 3, Threonine 6, and Threonine 11 are mu…

Promoter

CMV

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_GFAP_HyPer7_mito

Plasmid

#238966

Purpose

Mammalian astrocytes HyPer7 expression targeted to the mitochondrial matrix

Depositor

Dmitry Bilan

Article

Kotova et al Free Radic Biol Med. 2023 Nov 1;208:153-

Insert

HyPer7

Use

AAV

Tags

3x Cytochrome C Oxidase Subunit VIII presequense

Expression

Mammalian

Promoter

GFAP

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV_FLT3

Plasmid

#223566

Purpose

The plasmid is expressed FLT3 in mammalian cells.

Depositor

Hyun Woo Park

Article

Shin et al Mol Cancer. 2023 Nov 6;22(1):177. doi: 1

Insert

FLT3 wild type (FLT3 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Phi3-AURKB-PGK-mCherry

Plasmid

#153316

Purpose

Expresses AURKB and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

AURKB (AURKB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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TRBV14

Plasmid

#123449

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV14 (TRBV14 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV2

Plasmid

#123409

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV2 (TRBV2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV19

Plasmid

#123391

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV19 (TRAV19 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV8-4

Plasmid

#123377

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV8-4 (TRAV8-4 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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eTd-CBEm

Plasmid

#196602

Purpose

Mammalian expression of eTd-CBEm

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGEN6-Ubc5 Sc

Plasmid

#83234

Purpose

pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Inserts

Ubiqutin (UBC Human)
Ubc 5 (UBC5 Budding Yeast)
Uba 1

Tags

His

Expression

Bacterial

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pYO1025

Plasmid

#235705

Purpose

Expression of FL human VPS34 and human VPS15-ZZ in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Inserts

VPS34 (PIK3C3 Human)
VPS15 (PIK3R4 Human)

Tags

3xTEV-ZZ

Expression

Mammalian

Promoter

CAG and CMV

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgCasp8_#2-puro

Plasmid

#231978

Purpose

Knockout mouse Casp8

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgCasp8_#1-puro

Plasmid

#231977

Purpose

Knockout mouse Casp8

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV6-5

Plasmid

#123425

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV6-5 (TRBV6-5 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJT311 AAV-Rapamycin Inducible-NZF-Citrine

Plasmid

#202060

Purpose

Inducible AAV activator

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-NZF-Citrine

Use

AAV; Aav with reporter

Expression

Mammalian

Mutation

ITR deletion

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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eTd-CBEa

Plasmid

#196603

Purpose

Mammalian expression of eTd-CBEa

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-G614 (C-flag)

Plasmid

#156421

Purpose

Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Mutation

D614G

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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PB-PE SpRY

Plasmid

#179082

Purpose

NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…

Promoter

CAG

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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