Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Tuning Plant Promoters Using a Simple Split Luciferase Method to Assess Transcription Factor-DNA Interactions.
Cai YM, Witham S, Patron NJ
ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
196141pEPYCeGM0001Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tag
196142pEPYCeGM0002Gateway entry vector with Arabidopsis TGA2 CDS and c-terminal HiBiT tag
196143pEPYCeGM0009Gateway entry vector with Arabidopsis NLP6 CDS and c-terminal HiBiT tag
196144pEPYCeGM0010Gateway entry vector with Arabidopsis NLP7 CDS and c-terminal HiBiT tag
196145pEPYCeGM0011Gateway entry vector with Arabidopsis DREB26 CDS and c-terminal HiBiT tag
196146pEPYCeGM0012Gateway entry vector with Arabidopsis ANAC032 CDS and c-terminal HiBiT tag
196147pEPYCeGM0022Gateway entry vector with Arabidopsis ARF18 CDS and c-terminal HiBiT tag
196148pEPYCdKN0001Gateway destination vector for protein expression of 9xHis_AtTGA1_HiBit
196149pEPYCdKN0002Gateway destination vector for protein expression of 9xHis_AtTGA2_HiBit
196150pEPYCdKN0009Gateway destination vector for protein expression of 9xHis_AtNLP6_HiBit
196151pEPYCdKN0010Gateway destination vector for protein expression of 9xHis_AtNLP7_HiBit
196152pEPYCdKN0011Gateway destination vector for protein expression of 9xHis_AtDREB26_HiBit
196153pEPYCdKN0012Gateway destination vector for protein expression of 9xHis_AtNAC032_HiBit
196154pEPYCdKN0022Gateway destination vector for protein expression of 9xHis_AtARF18_HiBit
196155pEPYC0CM0515Phytobricks Level 0 part: MinSyn_301
196156pEPYC0CM0519Phytobricks Level 0 part: MinSyn_302
196157pEPYC0CM0520Phytobricks Level 0 part: MinSyn_303
196158pEPYC0CM0522Phytobricks Level 0 part: MinSyn_305
196159pEPYC0CM0513Phytobricks Level 0 part: MinSyn_308
196160pEPYC0CM0528Phytobricks Level 0 part: MinSyn_310
196161pEPYC0CM0529Phytobricks Level 0 part: MinSyn_311
196162pEPSW0CM0014Phytobricks Level 0 part: ANAC032 promoter 1kb upstream
196163pEPSW0CM0020Phytobricks Level 0 part: AtNLP6 promoter 1kb upstream
196164pEPSW0CM0018Phytobricks Level 0 part: AtARF18 promoter 1kb upstream
196165pEPSW0CM0350Phytobricks Level 0 part: AtTGA1_CDS
196166pEPYC1CB0597MoClo Level 1 luciferase reporter: MinSyn_301 reporter [MinSyn_301::TMV::LucF::flag::ocsT]
196167pEPYC1CB0601MoClo Level 1 luciferase reporter: MinSyn_302 reporter [MinSyn_302::TMV::LucF::flag::ocsT]
196168pEPYC1CB0602MoClo Level 1 luciferase reporter: MinSyn_303 reporter [MinSyn_303::TMV::LucF::flag::ocsT]
196169pEPYC1CB0604MoClo Level 1 luciferase reporter: MinSyn_305 reporter [MinSyn_305::TMV::LucF::flag::ocsT]
196170pEPYC1CB0595MoClo Level 1 luciferase reporter: MinSyn_308 reporter [MinSyn_308::TMV::LucF::flag::ocsT]
196171pEPYC1CB0607MoClo Level 1 luciferase reporter: MinSyn_310 reporter [MinSyn_308::TMV::LucF::flag::ocsT]
196172pEPYC1CB0608MoClo Level 1 luciferase reporter: MinSyn_311 reporter [MinSyn_308::TMV::LucF::flag::ocsT]
196173pEPSW1KN0351AtTGA1 expression cassette: CaMV35S::TMV::AtTGA1::35sT
196174pEPSW1KN0014MoClo Level 1 luciferase reporter: proANAC032::LucF::flag::notT
196175pEPSW1KN0020MoClo Level 1 luciferase reporter: proNLP6::LucN::flag::notT
196176pEPSW1KN0018MoClo Level 1 luciferase reporter: proARF18::LucN::flag::notT
196177pEPSW1KN0035MoClo Level 1 luciferase reporter: NOS::TMV::LucN::flag::nosT
196178pEPSW1KN0034MoClo Level 1 luciferase reporter: CaMV35S::TMV::LucF::flag::35sT
196179pEPOR1CB0068YFP expression cassette: CaMV35S::TMV::YFP::35sT
197517pEPSW0CM0022Phytobricks Level 0 part
197518pEPSW0CM0024Phytobricks Level 0 part
197519pEPSW0CM0025Phytobricks Level 0 part
197520pEPSW0CM0027Phytobricks Level 0 part
197521pEPSW0CM0029Phytobricks Level 0 part
197522pEPSW0CM0030Phytobricks Level 0 part
197523pEPSW0CM0031Phytobricks Level 0 part
197524pEPSW0CM0032Phytobricks Level 0 part
197526pEPSW0CM0112Phytobricks Level 0 part
197528pEPSW0CM0073Phytobricks Level 0 part
197529pEPSW0CM0074Phytobricks Level 0 part
197530pEPSW0CM0075Phytobricks Level 0 part
197531pEPSW0CM0076Phytobricks Level 0 part
197533pEPOZ0CM0138Phytobricks Level 0 part
197534pEPOZ0CM0139Phytobricks Level 0 part
197535pEPOZ0CM0137Phytobricks Level 0 part
197536pEPSW1KN0070MoClo Level 1 luciferase reporter
197537pEPSW1KN0016MoClo Level 1 luciferase reporter
197538pEPSW1KN0022MoClo Level 1 luciferase reporter
197539pEPSW1KN0024MoClo Level 1 luciferase reporter
197540pEPSW1KN0025MoClo Level 1 luciferase reporter
197541pEPSW1KN0027Level 1 gene expression cassette
197542pEPSW1KN0029Level 1 gene expression cassette
197543pEPSW1KN0030Level 1 gene expression cassette
197544pEPSW1KN0031Level 1 gene expression cassette
197545pEPSW1KN0032Level 1 gene expression cassette
197547pEPSW1KN0113Level 1 gene expression cassette
197549pEPOZ1KN0142Level 1 gene expression cassette
197550pEPOZ1KN0143Level 1 gene expression cassette
197551pEPOZ1KN0144Level 1 gene expression cassette
197552pEPOZ1KN0145Level 1 gene expression cassette
197554pEPOZ1KN0147Level 1 gene expression cassette
197555pEPOZ1KN0148Level 1 gene expression cassette

Antibodies from Article