We narrowed to 17,487 results for: puro

1. pCRISPaint-StrepTag II-PuroR

   Plasmid

   #80967

   Purpose

   CRISPaint gene tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   StrepTag II
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-ND-Cdt1-HA-Puro

   Plasmid

   #193767

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1-HA (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. H2B-2A-GFP-IRESpuro2

   Plasmid

   #183746

   Purpose

   Expresses H2B-2A-GFP

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   H2B (H2BC16P Human)
   

   Use

   Tags

   GFP with 2A protease cleavage site between H2B an…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. shCTL-mlpx-puro

   Plasmid

   #65232

   Purpose

   encodes a non-targeting control shRNA

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   non-coding shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBEG R2-iPuro-L3 #BGV205

   Plasmid

   #48987

   Purpose

   Supplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   puromycin resistance
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Expression

   Mutation

   Promoter

   NA

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pBABEpuro-ERBB2 C334S

   Plasmid

   #40983

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   C334S

   Promoter

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pLG1-puro-sgULK1-1

   Plasmid

   #109004

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ULK1-sgRNA #1 (ULK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSuper.Retro.Puro-WDR5 shRNA 2

   Plasmid

   #59975

   Purpose

   Knockdown of WDR5

   Depositor

   Debu Chakravarti

   Article

   Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

   Insert

   WDR5 shRNA
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLKO.1puro-shKIBRA-A

   Plasmid

   #40888

   Depositor

   Paul Reynolds

   Article

   Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

   Insert

   shKIBRA-A (WWC1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pLKO.1puro-shKIBRA-B

    Plasmid

    #40889

    Depositor

    Paul Reynolds

    Article

    Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

    Insert

    shKIBRA-B (WWC1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRetroSuper shSmad4J puro

    Plasmid

    #19152

    Depositor

    Joan Massague

    Article

    Padua et al Cell. 2008 Apr 4. 133(1):66-77.

    Insert

    small hairpin RNA against SMAD family member 4 (SMAD4 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pMSCV-puro-5A, 6A mMCL1-OM

    Plasmid

    #45818

    Depositor

    Joseph Opferman

    Article

    Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

    Insert

    5A, 6A mMCL-1-OM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Arginine to Alanine at 5th and 6th amino …

    Promoter

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pLVX-Tight-Puro_Myt1

    Plasmid

    #64845

    Purpose

    A lentiviral vector for the expression of mouse Myt1 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Myt1 (Myt1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_CMV:Flag-dKeNLuc_SV40:PuroR

    Plasmid

    #183046

    Purpose

    Expresses the FLAG-tagged dKeNLuc (Far-Red dKeima LumiFluor) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    dKeNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_CMV:Flag-KaNLuc_SV40:PuroR

    Plasmid

    #183047

    Purpose

    Expresses the FLAG-tagged KaNLuc (Far-Red LSSmKate2 LumiFluor) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    KaNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPaint-AviTag-PuroR

    Plasmid

    #80957

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    AviTag
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCF823_EFS-Cas9-PuroR_lenti

    Plasmid

    #138317

    Purpose

    Lenti expression of SpyCas9 with Puro selection

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB700-ACTC1-Puro

    Plasmid

    #128324

    Purpose

    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)

    Depositor

    Alejandro Chavez

    Insert

    gRNA against human ACTC1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX302-PTP-SL-V5 puro

    Plasmid

    #87774

    Purpose

    Expresses V5 tagged human PTP-SL

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    PTP-SL (PTPRR Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-puro-shPSMD2

    Plasmid

    #125779

    Purpose

    constitutive expression of a short-hairpin RNA targeting human PSMD2 (RNAi positive control)

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shPSMD2 (PSMD2 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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