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We narrowed to 1,854 results for: rigi

Showing: 961 - 980 of 1854 results

  1. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Expression
    Mutation
    Promoter
    TgTUB1 and Toxoplasma U6
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  4. pALS3-Ma-sfGFP-TAG150

    Plasmid
    #212121
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212121-plasmid-map-sequence-id-444186

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  7. pS3

    Plasmid
    #50596
    Purpose
    Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5
    Depositor
    Jay Keasling
    Article
    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8
    Inserts
    aroE
    aroD
    aroB
    aroG*
    ppsA
    tktA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    50596-plasmid-map-sequence-id-77162

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  9. T7-LacO-QF-T7-TetO-TetR_p15A

    Plasmid
    #171661
    Purpose
    Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    QF-T7-TetO-TetR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    171661-plasmid-map-sequence-id-342537

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  11. hfDECOR-F148A

    Plasmid
    #219546
    Purpose
    Expression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)
    Depositor
    Weixin Tang
    Article
    Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/
    Insert
    hfDECOR-F148A
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Changed TadA8e Phenylalanine 148 to Alanine
    Promoter
    CMV
    Available Since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    219546-plasmid-map-sequence-id-443106

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  12. hfDECOR-V106G

    Plasmid
    #219547
    Purpose
    Expression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)
    Depositor
    Weixin Tang
    Article
    Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/
    Insert
    hfDECOR-V106G
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Changed TadA8e Valine 106 to Glycine
    Promoter
    CMV
    Available Since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    219547-plasmid-map-sequence-id-443107

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Showing: 961 - 980 of 1854 results