We narrowed to 23,671 results for: CRISPR

1. pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710

   Plasmid

   #91913

   Purpose

   Expresses CaCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   CaCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEasyG2_zeo

   Plasmid

   #184913

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEasyG2_nat

   Plasmid

   #184914

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEasyG3_zeo

   Plasmid

   #184917

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106355

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   MYOD1 gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFETCh_BCL6_iso1

   Plasmid

   #104038

   Purpose

   Donor vector for 3' FLAG tag of human BCL6_iso1

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   BCL6_iso1 homology arms (BCL6 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mammalian

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_Tuba1b sgRNA / hSpCas9

   Plasmid

   #172835

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

   Plasmid

   #140204

   Purpose

   Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Step 1 of cloning c…

   Promoter

   Aspergillus fumigatus U3 (RNAPIII).

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJY-RpABE-FT_INF

   Plasmid

   #112874

   Purpose

   binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis

   Depositor

   Jin-Soo Kim, Jae-Young Yun

   Article

   Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

   Inserts

   RPS5A promoter
   ABE7.10
   U6-sgRNA targeting FT
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFETCh_GATAD1

    Plasmid

    #104036

    Purpose

    Donor vector for 3' FLAG tag of human GATAD1

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    GATAD1 homology arms (GATAD1 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mammalian

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-(BsaI)_CBh-Cas9

    Plasmid

    #135010

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Tags

    3xFLAG (N terminal on insert)

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. px458_2A_GFP_sgRNA_ELAVL2

    Plasmid

    #106107

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA targeting ELAVL2 (ELAVL2 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. gh172

    Plasmid

    #106833

    Purpose

    expression of gRNA targeting UGGT1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    UGGT1 (UGGT1 Human)
    

    Expression

    Mammalian

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. gh121

    Plasmid

    #106780

    Purpose

    expression of gRNA targeting ST8SIA1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST8SIA1 (ST8SIA1 Human)
    

    Expression

    Mammalian

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

    Plasmid

    #124859

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. gh49

    Plasmid

    #106722

    Purpose

    expression of gRNA targeting ST3GAL1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST3GAL1 (ST3GAL1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. gh42

    Plasmid

    #106716

    Purpose

    expression of gRNA targeting ST6GALNAC1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST6GALNAC1 (ST6GALNAC1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. gh45

    Plasmid

    #106708

    Purpose

    expression of gRNA targeting ST6GALNAC4

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST6GALNAC4 (ST6GALNAC4 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106353

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    D4Z4 gRNA-2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC024 - CgCas13a from Carnobacterium gallinarum DSM 4847

    Plasmid

    #91914

    Purpose

    Expresses CgCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    CgCas13a and guide expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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