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pAAV-DIO-Ef1a-TVA-2A-oG

Plasmid

#172359

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling specifically from cre-expressing neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-oG

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-haA3A-CBE-G-N

Plasmid

#220898

Purpose

AAV genome encoding the N-terminal of haA3A-CBE-G

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-N

Use

AAV

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB409

Plasmid

#74803

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

Plasmid

#191457

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluSnFR

Plasmid

#106123

Purpose

Indicator for glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR

Tags

Myc and PDGFR

Expression

Mammalian

Promoter

synapsin

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pETcLIC-GFP

Plasmid

#183514

Purpose

The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli

Depositor

Martin Högbom

Article

Grave et al Protein Expr Purif. 2022 Oct;198:106132.

Type

Empty backbone

Tags

10x His-tag and folding reporter GFP

Expression

Bacterial

Promoter

T7 lac

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112922

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Gateway entry vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dMisCas13b-SNAPf-NLS-3xFlag

Plasmid

#191536

Purpose

Overexpression of NLS-dMisCas13b-SNAPf-NLS-3xFlag in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dMisCas13b-SNAPf-NLS-3xFlag

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dMisCas13b-EGFP-NLS

Plasmid

#191359

Purpose

Overexpression of NLS-dMisCas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dMisCas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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mEos2-Integrin-Alpha2-N-18

Plasmid

#57389

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Integrin-Alpha2 (ITGA2 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Bxb1-GT_CAG_3xTurboGFP-NLS

Plasmid

#229777

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

3x TurboGFP

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-Lifeact-mRuby

Plasmid

#194339

Purpose

Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Lifeact

Tags

mRuby

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-Uricase

Plasmid

#182401

Purpose

Bacterial expression of Vesicle Nucleating peptide-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-uricase

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-E2_Crimson

Plasmid

#172377

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

E2_Crimson

Use

Neurotropic virus

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 SENP6

Plasmid

#14583

Depositor

Mary Dasso

Article

Mukhopadhyay et al J Cell Biol. 2006 Sep 25. 174(7):939-49.

Insert

SENP6 (SENP6 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only
pGWB521

Plasmid

#74863

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV CAG-DO-TC66T-mCherry

Plasmid

#175178

Purpose

Cre-off TVA-mCherry fusion protein with 66T mutation

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

TVA

Use

AAV

Tags

mCherry

Mutation

E66T

Promoter

CAG

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_PTPN22_Y-phosphatase

Plasmid

#110048

Purpose

Protein expression and purification of PTPN22_Y-phosphatase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PTPN22_Y-phosphatase (PTPN22 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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TRE3G-CTCF-WT-HA-MND-zeo

Plasmid

#232801

Purpose

Inducible over expression of wild type version of CTCF.

Depositor

Chunliang Li

Article

Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

Insert

CTCF (Ctcf Mouse)

Use

Lentiviral

Mutation

WT

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB509

Plasmid

#74851

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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