We narrowed to 15,995 results for: LEA

1. NFATc4-CRISPR-gRNA#exon5-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124290

   Purpose

   sgRNA against huiman NFATc4

   Depositor

   Katalin Josvay, Csaba Vizler

   Insert

   NFATc4 nuclear factor of activated T cells (NFATC4 Human)
   

   Use

   CRISPR

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

2. NFATc4-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124289

   Purpose

   sgRNA against human NFATc4

   Depositor

   Katalin Josvay, Csaba Vizler

   Insert

   NFATc4 nuclear factor of activated T cells (NFATC4 Human)
   

   Use

   CRISPR

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

3. WNK3 (WNK3B-c005)

   Plasmid

   #110262

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   WNK3 (WNK3 Human)
   

   Tags

   His-TEV

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. WNK3 (WNK3B-c001)

   Plasmid

   #110261

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   WNK3 (WNK3 Human)
   

   Tags

   His-TEV

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. WNK2 (WNK2A-c003)

   Plasmid

   #110250

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   WNK2 (WNK2 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7 - lacO

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. RF-227-UB-BMX

   Plasmid

   #79186

   Purpose

   Expression of the C-terminal fragment of BMX that is endogenously generated by caspase cleavage in mammalian cells

   Depositor

   Richard Fahlman

   Insert

   BMX (BMX Human)
   

   Tags

   3x Flag and ubiquitin

   Expression

   Mammalian

   Mutation

   delta 1-242

   Promoter

   CMV

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK289

   Plasmid

   #71553

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Expression

   Bacterial

   Mutation

   changes lysine-39 to alanine (please see deposito…

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGGW_SM_BglII

   Plasmid

   #49314

   Purpose

   Subcloning vector for Golden Gate-based site-directed mutagenesis; BglII and KpnI to release the insert

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b-EC12-129/138Avi

   Plasmid

   #47927

   Purpose

   Expresses E-cadherin EC12 with C9A, K129C/D138C mutations and avi tag in E.Coli.

   Depositor

   Tanja Kortemme

   Article

   Ritterson et al J Am Chem Soc. 2013 Aug 7.

   Insert

   E-Cadherin EC12
   

   Tags

   6xHis, Avi Tag, and TEV Cleavage

   Expression

   Bacterial

   Mutation

   Mutations C9A, K129C, D138C

   Promoter

   T7

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. GIMAP2

    Plasmid

    #28166

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GTPase IMAP family member 2 (GIMAP2 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    AIG1 domain only

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

11. Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] (Recombinant Antibody)

    Antibody

    #211756-rAb

    Purpose

    Recombinant mouse monoclonal Anti-SARS-CoV-2 nucleocapsid protein antibody

    Depositor

    Brian Geiss

    Article

    Terry et al Virology. 2021 Jun;558:28-37. doi: 10.10

    Recommended Applications

    Western Blot

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Not available to purchase

    Available Since

    March 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

12. Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] (Recombinant Antibody)

    Antibody

    #211757-rAb

    Purpose

    Recombinant mouse monoclonal Anti-SARS-CoV-2 nucleocapsid protein antibody

    Depositor

    Brian Geiss

    Article

    Terry et al Virology. 2021 Jun;558:28-37. doi: 10.10

    Recommended Applications

    Western Blot

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Not available to purchase

    Available Since

    March 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

13. spNW100

    Plasmid

    #173488

    Purpose

    Expresses spNW100 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW100
    

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    P1049L- please see depositor comments

    Promoter

    T7

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

    Plasmid

    #225754

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCURE

    Plasmid

    #23013

    Depositor

    James Bowie

    Article

    Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

    Insert

    I-CreI homing endonuclease
    

    Expression

    Bacterial

    Mutation

    I-CreI expression is controlled by the arabinose …

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pBAD-I-SceI

    Plasmid

    #60960

    Purpose

    Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

    Depositor

    Nikolaus Osterrieder

    Article

    Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

    Insert

    I-SceI restriction enzyme
    

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-AKAR4

    Plasmid

    #61619

    Purpose

    FRET-based biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    AKAR4
    

    Tags

    Cerulean and cpVenus[E172]

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a MDHM_YEAST

    Plasmid

    #204231

    Purpose

    Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHM_YEAST (MDH1 Budding Yeast)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Does not include transit peptide and includes a M…

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ECFP-TevS-YPET

    Plasmid

    #100097

    Purpose

    FRET-based ECFP-YPET reporter that is cleaved by TEV

    Depositor

    Jim Wells

    Article

    Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

    Insert

    ECFP-TevS-YPET
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. V5-APEX2-LMNA

    Plasmid

    #129275

    Purpose

    Expression of APEX2 at nuclear lamina

    Depositor

    Alice Ting

    Article

    Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

    Insert

    V5-APEX2-LMNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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