We narrowed to 12,435 results for: Vars;

1. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

   Plasmid

   #100834

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from CamKII promoter

   Depositor

   James M. Wilson

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   CamKII

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSETa mEos2

   Plasmid

   #20341

   Purpose

   bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

   Depositor

   Loren Looger

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   mEos-2
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. K44A HA-dynamin 1 pcDNA3.1

   Plasmid

   #34683

   Depositor

   Sandra Schmid

   Insert

   dynamin 1ba (DNM1L Human)
   

   Tags

   HA (variant)

   Expression

   Mammalian

   Mutation

   K44A

   Available Since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGR596

   Plasmid

   #213787

   Purpose

   Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

   Depositor

   Chang Liu

   Insert

   BadBoy2 TP-DNAP1
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Mutation

   See manuscript

   Available Since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBBi-Pur LaG17-synNotch-TetRVP64

   Plasmid

   #158133

   Purpose

   Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

   Depositor

   Neal Devaraj

   Insert

   LaG17-synNotch-TetRVP64
   

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBBi-Pur GFP-TM

   Plasmid

   #139185

   Purpose

   SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.

   Depositor

   Neal Devaraj

   Insert

   GFP-TM
   

   Use

   Transposon

   Expression

   Mammalian

   Mutation

   fused to membrane anchor

   Promoter

   EF1α

   Available Since

   March 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBBi-Pur LaG17-synNotch-Gal4VP64

   Plasmid

   #158135

   Purpose

   Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

   Depositor

   Neal Devaraj

   Insert

   LaG17-synNotch-Gal4VP64
   

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGR618

   Plasmid

   #213786

   Purpose

   Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

   Depositor

   Chang Liu

   Insert

   BB-Tv TP-DNAP1
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKT0128

   Plasmid

   #8729

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yEGFP
   

   Tags

   Codon optimized linker

   Expression

   Yeast

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMH1742

    Plasmid

    #160929

    Purpose

    Expression of soluble Sc octamer (Cse4)

    Depositor

    Stephen Harrison

    Inserts

    HHF1
    HTA1
    HTB1
    Cse4
    

    Tags

    6His-TEV, N-terminal 6His-TEV, and none

    Mutation

    codon-optimized for E. coli -- see provided seque…

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKT0209

    Plasmid

    #8730

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yEGFP
    

    Tags

    Codon optimized linker

    Expression

    Yeast

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid

    #125199

    Purpose

    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Insert

    Lyn-AktAR2-EV
    

    Use

    Transposon

    Tags

    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE-60NA-mEGFP

    Plasmid

    #160459

    Purpose

    Encodes a monomeric enhanced GFP variant

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    mEGFP
    

    Promoter

    T5

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSAN-QUEEN 84BP

    Plasmid

    #129378

    Purpose

    a variation of QUEEN ATP sensor

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN TogBP (84BP) N/A
    

    Expression

    Bacterial

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR hH1-ARRET

    Plasmid

    #111382

    Purpose

    ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminator

    Depositor

    Klaus Holzmann

    Insert

    hH1-ARRET
    

    Use

    Gateway entry cloning

    Promoter

    hH1

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR8GW-HA-Wnk

    Plasmid

    #203967

    Purpose

    HA-Wnk basic construct

    Depositor

    Andreas Jenny

    Insert

    Wnk (most similar to RO variant) (Wnk Fly)
    

    Use

    Unspecified

    Tags

    HA

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-lambdaN-HA-DmMTR-4_AA

    Plasmid

    #148215

    Purpose

    Insect Expression of DmMTR-4

    Depositor

    Elisa Izaurralde

    Insert

    DmMTR-4 (l(2)35Df Fly)
    

    Expression

    Insect

    Mutation

    7 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1B-EGFP-DmGW182_777-1348_S

    Plasmid

    #147445

    Purpose

    Insect Expression of DmGW182_777-1348

    Depositor

    Elisa Izaurralde

    Insert

    DmGW182_777-1348 (gw Fly)
    

    Expression

    Insect

    Mutation

    one non silent mutation N799S compared to the seq…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1A-DmXRN1-A1349P-V5His6_Q

    Plasmid

    #147251

    Purpose

    Insect Expression of DmXRN1-A1349P

    Depositor

    Elisa Izaurralde

    Insert

    DmXRN1-A1349P (pcm Fly)
    

    Expression

    Insect

    Mutation

    one silent mtuation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAc5.1B-lambdaN-HA-DmPop2a-D53AE55A-V5His6_G

    Plasmid

    #146370

    Purpose

    Insect Expression of DmPop2a-D53AE55A

    Depositor

    Elisa Izaurralde

    Insert

    DmPop2a-D53AE55A (Pop2 Fly)
    

    Expression

    Insect

    Mutation

    one non silent variation I164F compared to the se…

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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