We narrowed to 11,626 results for: VARS

1. pDL26_Cas9-His Δcys M1C/E532C

   Plasmid

   #206287

   Purpose

   Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, E532C, C574S

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDL22_Cas9-His Δcys E532C

   Plasmid

   #206283

   Purpose

   Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG1.1-HA/Ppp1cc2

   Plasmid

   #199626

   Purpose

   Expression vector of mouse PPP1CC2. HA tag at N-terminus.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   Ppp1cc mRNA (variant X1) (Ppp1cc Mouse)
   

   Tags

   HA-tag

   Promoter

   CAG

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1A-DmXRN1-dsRNAres-V5His6_N

   Plasmid

   #147027

   Purpose

   Insect Expression of DmXRN1-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

   Insert

   DmXRN1-dsRNAres (pcm Fly)
   

   Expression

   Insect

   Mutation

   one silent mtuation compared to the sequence give…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAFMW-Nbr-CM

   Plasmid

   #188585

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler Catalytic mutant
   

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   deleted first ATG; D435A [GAT=>GCC]; E435A [GA…

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAFMW-Nbr[N1-383]

   Plasmid

   #188586

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler N-terminal domain
   

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   deleted first ATG

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAFMW-Nbr[C399-625]

   Plasmid

   #188587

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler C-terminal domain
   

   Tags

   FLAG-Myc

   Expression

   Insect

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5.1B-lambdaN-HA-DmXRN1_A

   Plasmid

   #145879

   Purpose

   Insect Expression of DmXRN1

   Depositor

   Elisa Izaurralde

   Article

   Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

   Insert

   DmXRN1 (pcm Fly)
   

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CD44U-c011

   Plasmid

   #175454

   Purpose

   Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Tags

   AviTag-His6

   Expression

   Mammalian

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJH580

    Plasmid

    #172164

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (pylBCD 3f2v2+B); AraC
    

    Expression

    Bacterial

    Mutation

    PylB(N61S, E84K, E122K, E178K, G188R, E251D, G332…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJH61

    Plasmid

    #172143

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] pylS; PproK pylT
    

    Expression

    Bacterial

    Mutation

    gIII(P29*); [strong promoter eG, strong RBS SD8] …

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSUMO chicken-cPLA2-C2-domain

    Plasmid

    #170753

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Chicken)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. P302-FBP_1

    Plasmid

    #139692

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    p35S_Short_TMV 5':AsNpga:tAtug7, AtUbi10:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. P313-FBP_8

    Plasmid

    #139699

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing H3H

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp, pAtuMas:Nn Luz:trbcSE9
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-Citroff1-R251

    Plasmid

    #134307

    Purpose

    Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citroff1 R251A
    

    Tags

    6-His tag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-Citroff1-S245

    Plasmid

    #134302

    Purpose

    Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citroff1 S245A
    

    Tags

    6-His tag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MYMH 391

    Plasmid

    #138059

    Purpose

    Encodes Anderson promoter variant using mRFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    J23101- pSEVA-331Bb- RFP
    

    Expression

    Bacterial

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MYMH 392

    Plasmid

    #138053

    Purpose

    Encodes Anderson promoter variant using mRFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    J23102- pSEVA-331Bb- RFP
    

    Expression

    Bacterial

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MYMH 67

    Plasmid

    #138048

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-11AT-GFP
    

    Expression

    Bacterial

    Mutation

    AAA GAG GAG ATA

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. MYMH 49

    Plasmid

    #138039

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-8AT-GFP
    

    Expression

    Bacterial

    Mutation

    AAA GAG GTG AAA

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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