We narrowed to 25,359 results for: grna

1. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

   Plasmid

   #73543

   Purpose

   Expression of sgRNA targeting LINE-1 and TagBFP

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   LINE-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. px459-Flag-Mcm2 targeting sgRNA

   Plasmid

   #186937

   Purpose

   Genomic targeting of Flag tag at Mcm2 N-terminal

   Depositor

   Zhiguo Zhang

   Article

   Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

   Insert

   Flag-Mcm2 targeting sgRNA (Mcm2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

   Plasmid

   #161924

   Purpose

   To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   sgRNA targeting RDH11 exon 2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB700 lenti gRNA KanR neo_zhang2.0

   Plasmid

   #167917

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9(1.1)-ABL1-gRNA1-exon4

   Plasmid

   #163322

   Purpose

   eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

   Depositor

   Val Brunton

   Article

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Insert

   ABL1 gRNA 1_Exon4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

6. eSpCas9(1.1)-ABL1-gRNA2-exon4

   Plasmid

   #163323

   Purpose

   eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

   Depositor

   Val Brunton

   Article

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Insert

   ABL1 gRNA 2_Exon4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

7. 330-BFP-hCYP3A4-enhancer-R-sgRNA

   Plasmid

   #176841

   Purpose

   Targeting human CYP3A4 proximal enhancer right boundary. sgRNA expressing cells could be FACS sorted by BFP expression.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Human CYP3A4 proximal enhancer right boundary
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 330-cherry-hCYP3A4-enhancer-L-sgRNA

   Plasmid

   #176840

   Purpose

   Targeting human CYP3A4 proximal enhancer left boundary. sgRNA expressing cells could be FACS sorted by cherry expression.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Human CYP3A4 proximal enhancer left boundary
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330.puro sgRNA KGA Stop1

   Plasmid

   #110405

   Purpose

   CRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistance

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2ABFP-W

    Plasmid

    #163170

    Purpose

    Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2AmCherry-W

    Plasmid

    #163172

    Purpose

    Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNTI733 pRPR1(TetO)-RPC31-sgRNA

    Plasmid

    #164913

    Purpose

    For yeast genomic integration of sgRNA against RPC31

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    pRPR1(TetO)-RPC31-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RPR1

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNTI732 pRPR1(TetO)-HTS1-sgRNA

    Plasmid

    #164912

    Purpose

    For yeast genomic integration of sgRNA against HTS1

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    pRPR1(TetO)-HTS1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RPR1

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUPD2 sgRNA sCRNA 2.0 (GB2461)

    Plasmid

    #160593

    Purpose

    Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNA sCRNA 2.0
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-5U6-sgRNAs-hsyn-EGFP

    Plasmid

    #112213

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    sgRNAs
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

    Plasmid

    #133791

    Purpose

    U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    St1Cas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V1 sgRNA architecture from Carter et al. biorxiv …

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

    Plasmid

    #133792

    Purpose

    U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    St3Cas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    sgRNA architecture from Muller et al. Molecular T…

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

    Plasmid

    #133343

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_gcrA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid

    #133345

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_blaA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZDonor-U6-myogenin stop codon gRNA

    Plasmid

    #69549

    Purpose

    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Insert

    Myogenin C terminus-targeting guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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