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1208 p3e-long etv2 3’UTR

Plasmid

#49010

Purpose

Zebrafish Long length etv2 3' UTR in gateway 3' Entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Mutation

Longest 3' UTR

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-GST

Plasmid

#39286

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only

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psbA2-PHLS (b)

Plasmid

#52307

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

psbA2

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5

Plasmid

#52540

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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M26V DJ-1 pET15b

Plasmid

#60688

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26V DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26V

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Hmga2 ORFtr

Plasmid

#14794

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 ORFtr (Hmga2 Mouse)

Expression

Mammalian

Mutation

Truncated ORF, but intact miRNA complementary sit…

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pJW3.42

Plasmid

#62866

Purpose

Yeast two-hybrid bait vector expressing LexA-PPTR-1 fusion

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

PPTR-1/pLexA-N bait

Use

Two-hybrid bait vector

Tags

LexA

Expression

Yeast

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1-internal6xHis (1-440)

Plasmid

#60391

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4), amino a…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
psbA2-Ptrc-PHLS (d)

Plasmid

#52309

Purpose

Replaces the psbA2 gene in Synechocystis with the Ptrc promoter - PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pTAtUbq3 (GB0219)

Plasmid

#68192

Purpose

Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq3 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shPIK3CD.v2 puro

Plasmid

#58707

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only
pFP122

Plasmid

#21669

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pMXs-B3

Plasmid

#31707

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-20b (Mir20b Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pRY1004

Plasmid

#21788

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Dig1-YFP (DIG1 Budding Yeast, A. victoria)

Tags

YFP

Expression

Yeast

Mutation

contains 5' 1000bp UTR (Dig1 promoter) Di…

Available Since

Sept. 18, 2009

Availability

Academic Institutions and Nonprofits only

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pGEM-rat Fcepsilon RI gamma

Plasmid

#8375

Depositor

Jean Kinet

Article

Blank et al Nature 1989 Jan 12;337(6203):187-9.

Insert

rat Fc epsilon RI gamma cDNA (Fcer1g Rat)

Use

Beta-gal

Expression

Bacterial

Available Since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL5B

Plasmid

#67379

Purpose

Expression of native hArl5b in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5B (ARL5B Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2 S274A

Plasmid

#44107

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S274A (TRAF2 Human)

Tags

3xMyc

Expression

Mammalian

Mutation

Serine 274 to Alanine

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
CCD+BATS

Plasmid

#30499

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Barkor (ATG14 Human)

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

71-180aa (CCD) + 413-492aa (BATS)

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pNUT N6His mICA NG

Plasmid

#70162

Purpose

Expresses N-His tagged nonglycosylated mouse inhibitor of carbonic anhydrase

Depositor

Ross MacGillivray, Anne Mason

Article

Wang et al Biochem J. 2007 Aug 15;406(1):85-95.

Insert

mouse inhibitor of carbonic anhydrase

Tags

N-terminal signal peptide, 4 aa link, 6 His, Fact…

Expression

Mammalian

Mutation

Asn470Asp and Asn645Asp

Promoter

SV40

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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FCIV1-GR SS287AA/S155A

Plasmid

#72700

Purpose

Lentiviral expression of GR-2A phosphodeficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Lambert et al Mol Cell Biol. 2013 Sep;33(18):3700-14.

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

SS287AA/S155A

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only