Addgene

We narrowed to 2,447 results for: PGK

Showing: 981 - 1000 of 2447 results

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pKI-cjT-tdTomato-PGK-Neo

Plasmid

#186173

Purpose

Knock-in gene targeting in marmoset cells at TBXT locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjOSR1-EGFP-PGK-Puro

Plasmid

#186170

Purpose

Knock-in gene targeting in marmoset cells at OSR1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

EGFP

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjSF1-tdTomato-PGK-Neo

Plasmid

#186172

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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PL752-hGH-polyA-PGK-puroR

Plasmid

#191682

Purpose

Donor plasmid backbone PGK-puroR

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-LexA-CIB1-biLINuS

Plasmid

#192607

Purpose

Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjSF1-hCD271-PGK-Neo

Plasmid

#186171

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

hCD271

Use

Marmoset targeting

Tags

Expression

Mutation

human codon-optimized

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXL2-EGFP-PGK-Puro

Plasmid

#186167

Purpose

Knock-in gene targeting in marmoset cells at FOXL2 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

EGFP

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2ABFP-W

Plasmid

#163175

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-W

Plasmid

#163174

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

Plasmid

#73542

Purpose

Expression of sgRNA targeting LINE-1 and TagBFP

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

LINE-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

Plasmid

#73543

Purpose

Expression of sgRNA targeting LINE-1 and TagBFP

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

LINE-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

Plasmid

#169550

Purpose

Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-SNAP-MDMUT-OCT4

Plasmid

#115692

Purpose

Constitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a non-functional mitotic degron (MDMUT) for control

Depositor

David Suter

Article

Friman et al Elife. 2019 Dec 3;8. pii: 50087. doi: 10

Insert

SNAP-MDMUT-OCT4

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2304-Tier1-Tactb-PPGK-NLuc_TB

Plasmid

#169549

Purpose

Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2308-Tier1-pGL3-PPGK-NLuc_TB

Plasmid

#169551

Purpose

Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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Phage-PGK-Flag-HA-FKBP-T(G177D)

Plasmid

#181735

Purpose

for lentiviral expression of brachyury ORF in mammalian cells

Depositor

Charles Lin

Article

Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

Insert

T (brachyury)

Use

Lentiviral

Tags

Expression

Mutation

a silent mutation in the PAM site of an sgRNAt ta…

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pHR: pPGK PYL1-sfGFP-HP1AI165E

Plasmid

#174114

Purpose

Expression of PYL1-sfGFP-HP1AI165E

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

PYL1-sfGFP-HP1AI165E

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHR: pPGK PYL1-sfGFP-HP1Adel72

Plasmid

#174113

Purpose

Expression of PYL1-sfGFP-HP1Adel72

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

PYL1-sfGFP-HP1Adel72

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-V12-puro

Plasmid

#156171

Purpose

Lentiviral vector for expression of RAP2A-V12, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-N17-puro

Plasmid

#156172

Purpose

Lentiviral vector for expression of RAP2A-N17, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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