Addgene

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pSC300

Plasmid

#31820

Depositor

Yossef Av-Gay

Article

Cowley et al Gene. 2001 Feb 21;264(2):225-31.

Insert

GFP (lacking ATG start codon)

Use

Tags

GFP (lacking ATG start codon)

Expression

Bacterial

Mutation

Promoter

SOD

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MVK mazei

Plasmid

#108947

Purpose

heterologous expression of MVK of M. mazei in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate kinase (MVK)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pStA0::crtI

Plasmid

#114343

Purpose

crtI in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtI (codon optimised)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-HMGS

Plasmid

#108945

Purpose

heterologous expression of HMGS of E. faecalis in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

HMG-CoA synthase (HMGS)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

A110G

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLEW100cre-EP1-6G

Plasmid

#33344

Depositor

George Cross

Article

Scahill et al Mol Biochem Parasitol. 2008 Jan;157(1):7

Insert

cre recombinase

Use

Trypanosoma brucei expression vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MVK jannaschii

Plasmid

#108948

Purpose

heterologous expression of MVK of M. jannaschii in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate kinase (MVK)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pStA0::eyfp

Plasmid

#114351

Purpose

efyp in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

efyp (codon optimised)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::J23116

Plasmid

#114331

Purpose

Promoter J23116 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

Promoter J23116

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::RBSc42

Plasmid

#114338

Purpose

RBS RBSc42 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

RBS RBSc42

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::L3S2P55

Plasmid

#114339

Purpose

Terminator L3S2P55 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

Terminator L3S2P55

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::crtW

Plasmid

#114347

Purpose

crtW in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtW (codon optimised)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::crtE

Plasmid

#114345

Purpose

crtE in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtE (codon optimised)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJK_proJK1_LGK

Plasmid

#81160

Purpose

Expresses levoglucosan kinase with promoter proJK1

Depositor

Timothy Whitehead

Article

Klesmith et al ACS Synth Biol. 2015 Sep 14.

Insert

Levoglucosan kinase wild-type

Use

Tags

His6x

Expression

Bacterial

Mutation

Promoter

proJK1

Available since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pGYL1_pPromALB_FireflyLuc

Plasmid

#220282

Purpose

Firefly luciferase with minimal Albumin promoter without enhancer; enhancer of interest can be cloned into MCS

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-MBP_D

Plasmid

#146120

Purpose

Insect Expression of MBP

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

MBP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDK365

Plasmid

#134195

Purpose

Retroviral plasmid with SKAP insert

Depositor

Iain Cheeseman

Insert

SKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)

Use

Retroviral

Tags

LAP (GFP)

Expression

Mammalian

Mutation

Alternate Start, G5 siRNA hardened

Promoter

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pStA0::crtB

Plasmid

#114344

Purpose

crtB in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtB (codon optimised)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::dxs

Plasmid

#114346

Purpose

dxs in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

dxs (codon optimised)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::crtZ

Plasmid

#114348

Purpose

crtZ in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtZ (codon optimised)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::lcyB

Plasmid

#114349

Purpose

lcyB in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

lcyB (codon optimised)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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