We narrowed to 3,278 results for: multiplex

1. pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31

   Plasmid

   #165887

   Purpose

   G418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   All-in-one FC31 RMCE Cassette
   

   Use

   Synthetic Biology

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTrans_9xD3B_Cluc_Norm

   Plasmid

   #68415

   Purpose

   CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   Cypridina Luciferase-2A-Venus YFP
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   YFP: M153T,V163A,S175G ("Venus") YFP Va…

   Promoter

   minimalCMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9v2

   Plasmid

   #103140

   Purpose

   TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

   Depositor

   Jens Nielsen

   Article

   Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

   Insert

   dCas9-VPR
   

   Expression

   Yeast

   Mutation

   Removed scaffold gRNA from original plasmid

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330A_D10A-1x7

   Plasmid

   #58777

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. hCMV-IE1:GLuc:bgH

   Plasmid

   #135294

   Purpose

   Transcriptional unit for constitutive expression of GLuc in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:GLuc:bGH
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Mammalian

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ151

   Plasmid

   #69302

   Purpose

   Gateway entry vector with pcoCas9D10A

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pcoCas9D10A (Plant codon-optimized)
   

   Use

   CRISPR

   Tags

   2XFLAG

   Mutation

   D10A mutation; NLS fusion

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Plasmid

   #163062

   Purpose

   Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   QuasAr2
   

   Use

   Gateway entry vector

   Tags

   mOrange2

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmTFP1-ShadowG

   Plasmid

   #99867

   Purpose

   Measure FRET between mTFP1 and ShadowG

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   ShadowG
   

   Tags

   mTFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLH-sgRNA1-MS2-PP7

   Plasmid

   #75392

   Purpose

   sgRNA1-MS2-PP7

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-2XMS2-PP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pR6Kg-Alpha1

    Plasmid

    #165862

    Purpose

    Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCGB

    Plasmid

    #135283

    Purpose

    GBPart - CGB Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Click Beetle Green Luciferase
    

    Use

    Luciferase and Synthetic Biology

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWS5394-CRISPRai-MET17

    Plasmid

    #182833

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - MET17 marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVimentin-miRFP670nano

    Plasmid

    #127436

    Purpose

    Vimentin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    Vimentin-miRFP670nano
    

    Tags

    miRFP670nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ132D

    Plasmid

    #69288

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRU323

    Plasmid

    #167690

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::Cas9i
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. nCas9-NG_P2A-mCherry

    Plasmid

    #219934

    Purpose

    Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-NG_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRU292

    Plasmid

    #167687

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::Cas9i
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pR6Kg-Omega1

    Plasmid

    #165864

    Purpose

    Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ140

    Plasmid

    #99895

    Purpose

    Empty vector with attL5-attL2 recombination sites for Gateway Cloning

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR and TALEN

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMyosin-miRFP670nano

    Plasmid

    #127431

    Purpose

    LC-Myosin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano-LC-Myosin
    

    Tags

    miRFP670nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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