We narrowed to 4,785 results for: pcr

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pMH5

Plasmid

#52531

Purpose

expresses FLP recombinase in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

FLP recombinase (flp Budding Yeast)

Expression

Insect

Promoter

ubiquitin

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pAc5 HA3-eDHFR-T2A-neo

Plasmid

#86396

Purpose

for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

Depositor

David Bentley

Article

Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

Insert

HA3 eDHFR T2A neo

Use

CRISPR

Tags

HA3 eDHFR T2A neo

Mutation

R12Y, G67S and Y100I mutations in E coli DHFR

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pG-HIV-LRT

Plasmid

#104592

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

HIV-1 late reverse transcripts

Use

Cloning vector

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1-3HA

Plasmid

#41609

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, TRP1 reverse orientation, and yeast ADH1 te…

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1-GFP

Plasmid

#41615

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP(S65T), TRP1 reverse orientation, and yeast AD…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pAAH1

Plasmid

#41825

Purpose

Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.

Depositor

Melissa Moore

Article

Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Type

Empty backbone

Use

Pcr template

Expression

Bacterial and Yeast

Promoter

T7, TEF

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1-GST

Plasmid

#41612

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GST, TRP1 reverse orientation, and yeast ADH1 ter…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pKF251

Plasmid

#52532

Purpose

Double-GFP reporter for DNA cleavage

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Double-GFP

Expression

Insect

Promoter

ubiquitin

Available Since

April 25, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-HphMX6

Plasmid

#240973

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HphMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-C.a.URA3

Plasmid

#240970

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; C.a.URA3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8d∆60-TRP1

Plasmid

#240968

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; TRP1 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-K.I.LEU2

Plasmid

#240969

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; K.l.LEU2 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-NatMX6

Plasmid

#240972

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; NatMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-His3MX6

Plasmid

#240971

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HIS3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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AbVec1.1-IGLC2-XhoI

Plasmid

#99575

Purpose

Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

Depositor

Hedda Wardemann

Article

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-13Myc-kanMX6

Plasmid

#39294

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

13Myc, A. gossypii translation elongation factor …

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP(S65T)-kanMX6

Plasmid

#39292

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-His3MX6-PGAL1

Plasmid

#41607

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-13Myc-His3MX6

Plasmid

#41602

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

13Myc, A. gossypii translation elongation factor …

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-3HA-kanMX6

Plasmid

#39295

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

3HA, A. gossypii translation elongation factor 1a…

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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