We narrowed to 4,697 results for: pcr

1. pKF251

   Plasmid

   #52532

   Purpose

   Double-GFP reporter for DNA cleavage

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   Double-GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   ubiquitin

   Available since

   April 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. AbVec1.1-IGLC2-XhoI

   Plasmid

   #99575

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. STAGR_gRNAScaffold_h7SK

   Plasmid

   #102842

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_h7SKpromoter
   

   Use

   As pcr template

   Tags

   Expression

   Mutation

   Promoter

   h7SK

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-GFP(S65T)-kanMX6

   Plasmid

   #39292

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Aug. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-13Myc-His3MX6

   Plasmid

   #41602

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   13Myc, A. gossypii translation elongation factor …

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-3HA-kanMX6

   Plasmid

   #39295

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   3HA, A. gossypii translation elongation factor 1a…

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-13Myc-kanMX6

   Plasmid

   #39294

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   13Myc, A. gossypii translation elongation factor …

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. STAGR_gRNAScaffold_hU6

   Plasmid

   #102840

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_hU6promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-GST-kanMX6

   Plasmid

   #39293

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-GST-His3MX6

    Plasmid

    #41604

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-kanMX6-PGAL1

    Plasmid

    #41605

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-His3MX6-PGAL1

    Plasmid

    #41607

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKF257

    Plasmid

    #52533

    Purpose

    expresses I-SceI nuclease in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Tags

    HA and NLS

    Expression

    Insect

    Mutation

    Promoter

    tubulin

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR-SUMO1

    Plasmid

    #114388

    Purpose

    pDONR vector with SUMO1 gene

    Depositor

    Vincent Dion

    Article

    Aeschbach et al Sci Rep. 2017 Dec 21;7(1):18026. doi: 10

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q29H compared with NCBI reference NP_003343.1

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHyg-His6

    Plasmid

    #212776

    Purpose

    C-terminal PCR-based tagging with His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMCRW2

    Plasmid

    #127380

    Purpose

    pBC with 4 repeats of 80bp DNA template

    Depositor

    Richard C. Willson

    Article

    Chavan et al Analyst. 2020 Jul 21;145(14):4942-4949.

    Insert

    4 repeats of 80 bp DNA template sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pG-HIV-SIN-2LTR

    Plasmid

    #104591

    Purpose

    Standard for qPCR

    Depositor

    Kristine Yoder

    Article

    Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

    Insert

    2LTR circle junction of self-inactivating HIV-based vectors
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD-TRP32

    Plasmid

    #71730

    Purpose

    inducible expression of TRP32 in E.coli

    Depositor

    Jere McBride

    Article

    Luo et al Infect Immun. 2008 Apr;76(4):1572-80. do

    Insert

    TRP32
    

    Use

    Tags

    6xHIS and Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pFA6a-GFP(S65T)-His3MX6

    Plasmid

    #41598

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. All_in_one_CRISPR/Cas9_LacZ

    Plasmid

    #74293

    Purpose

    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

    Depositor

    Lynne Postovit

    Article

    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

    Inserts

    LacZ-alpha
    Cas9
    mCherry
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Lac promoter, SV40, and T7 promoter

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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