We narrowed to 11,584 results for: Vars;

1. pFX-EGFP-KRasCT

   Plasmid

   #175833

   Purpose

   Express EGFP with plasma membrane-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with plasma membrane-targeting sequence
   

   Tags

   C-terminal hypervariable region of K-Ras 4B

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmAGO1

   Plasmid

   #22420

   Depositor

   Elisa Izaurralde

   Article

   Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

   Insert

   Argonaut-1, variant A (AGO1 Fly)
   

   Use

   Drosophila expression

   Tags

   lambdaN-HA

   Available Since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-hRedOpsin-TGFB3

   Plasmid

   #164429

   Purpose

   AAV plasmid expressing TGFB3 in photoreceptors

   Depositor

   Connie Cepko

   Article

   Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

   Insert

   Tgfb3 (Tgfb3 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   human red opsin

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. bdSUMO-R9-GFP-HiBit

   Plasmid

   #170990

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-R9-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. 459 pME-etv2

   Plasmid

   #49002

   Purpose

   full length coding sequence for zebrafish etv2 gene in gateway middle entry vector

   Depositor

   Nathan Lawson

   Article

   Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

   Insert

   ets variant gene 2 (etsrp Zebrafish)
   

   Use

   Gateway middle entry

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET41a iGluf

   Plasmid

   #119833

   Purpose

   Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluf
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E25D

   Promoter

   T7

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTEV5_ RpMatB_4

   Plasmid

   #78208

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak binding

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

   Insert

   matB (His6 C106A R286C T303A Q457C K488A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A R286C T303A Q457C K488A

   Promoter

   T7

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTEV5_ RpMatB_3

   Plasmid

   #78207

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

   Insert

   matB (His6 C106A T167A R286C Q457C K488A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A T167A R286C Q457C K488A

   Promoter

   T7

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS U6 WIP RNAi

   Plasmid

   #11774

   Depositor

   Jack Strominger

   Article

   Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

   Insert

   WIP RNAi (WIPF1 Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

10. Split-RESCUE-N

    Plasmid

    #170153

    Purpose

    AAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    NES-MCP-RESCUE-DDN
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-MA-VN

    Plasmid

    #123283

    Purpose

    Mammalian expression plasmid for matrix domain of HIV-1 Gag fused to the N-terminal half of split fluorescent Venus (VN)

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    Matrix
    

    Tags

    VN: N-terminus of split Venus (a.a. V1–A154) I152…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET41a iGlul

    Plasmid

    #119832

    Purpose

    Production of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGlul
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    T92A

    Promoter

    T7

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ERroGFP_C147S_pCDNA3

    Plasmid

    #48630

    Purpose

    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERroGFP
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C147S

    Available Since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-CMV-H2B-FusionRed

    Plasmid

    #164430

    Purpose

    AAV plasmid expressing nuclear-localized FusionRed fluorophore

    Depositor

    Connie Cepko

    Article

    Wang et al Mol Ther Methods Clin Dev. 2020 Oct 10;1

    Insert

    H2B-FusionRed
    

    Use

    AAV

    Tags

    H2B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFX-Venus-KRasCT

    Plasmid

    #175834

    Purpose

    Express Venus with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with plasma membrane-targeting sequence
    

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-EGFP-Rheb-HVR HRas-IP

    Plasmid

    #13833

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

    Insert

    Rheb-HVR HRas (Rheb Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminal of Rheb and hyper variable region (HVR…

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

17. ERrefGFP1_pCDNA3

    Plasmid

    #48635

    Purpose

    Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERrefGFP1
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C147S,C204Q

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. C. difficile 16S toehold switch sensor

    Plasmid

    #110704

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    C. difficile 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEL065

    Plasmid

    #124896

    Purpose

    For genome editing in plant by SpCas9 variant(SpCas9-NGv1)

    Depositor

    Yong Zhang

    Article

    Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TALEN-CCR5L18-CtermCan

    Plasmid

    #51438

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with canonical C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) Can C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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