We narrowed to 12,437 results for: yeast

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pBS-SKII-3XHA-eDHFR-URA

Plasmid

#188927

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-URA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Phleomycin

Plasmid

#188925

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Phleomycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Hygromycin

Plasmid

#188924

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Hygromycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Phleomycin

Plasmid

#188920

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Phleomycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Hygromycin

Plasmid

#188919

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Hygromycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-TalO8 (Blank-CoTrIP)

Plasmid

#178303

Purpose

Blank CoTrIP plasmid

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Type

Empty backbone

Tags

NA

Expression

Yeast

Promoter

NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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KBB521

Plasmid

#185103

Purpose

1st 960 amino acids (C-terminal truncation) of Pom152 fused with HA and GFP

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Tags

GFP

Expression

Yeast

Mutation

POM152(1-960)-HA-GFP

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJL32

Plasmid

#174066

Purpose

Encodes for CIB1 fused to VP16 in S. cerevisiae. Part of optogenetic system.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGal1-LacO-3NLS-CIB1-VP16-tADH1

Tags

FLAG

Expression

Yeast

Promoter

pGal1

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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RP51A-No5'ss-NoBS-NoPseudoBS

Plasmid

#100987

Purpose

RP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BS

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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RP51A-NoBS-NoPseudoBS

Plasmid

#100986

Purpose

RP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BS

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

BS mutated to GTTAGTG, pseudo BS mutated to GTTTG…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJL45

Plasmid

#174067

Purpose

Expresses CIB1 in S. cerevisiae. Includes inducible lac operon.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGal1-LacO-3NLS-CIB1-tADH1

Tags

FLAG

Expression

Yeast

Promoter

pGal1

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJL1

Plasmid

#174070

Purpose

Expresses VP16-CIB1 in S. cerevisiae. Part of optogenetic system.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGal-Olac-3xNLS-VP16-CIB1-tADH1

Tags

FLAG

Expression

Yeast

Promoter

pGal1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-mNeonGreen (pJJF003)

Plasmid

#173459

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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U6-U57A

Plasmid

#169927

Purpose

WT U6 with a U57A mutation

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57A

Expression

Bacterial and Yeast

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pVG97

Plasmid

#164849

Purpose

Plasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t construct

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

Part of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA

Expression

Yeast

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI743 dual-barcoded gRNA parent vector

Plasmid

#164915

Purpose

CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GXB

Plasmid

#160926

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCL21-decoder

Plasmid

#159448

Purpose

Insert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pact1-lexA-ER-B112-phosphodegron-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

act1

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCL33-decoder

Plasmid

#159451

Purpose

Insert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pfus1mut-tetR-nls-malE-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfus1mut

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCL38-decoder

Plasmid

#159452

Purpose

Insert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pfus1tet-LexA-ER-B112-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfus1tet

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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