We narrowed to 18,366 results for: Mut

1. pAc5.1B-lambdaN-HA-DmSmaug-K606AR609A-V5His6_C

   Plasmid

   #146016

   Purpose

   Insect Expression of DmSmaug-K606AR609A

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug-K606AR609A (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   10 silent mutations and a deletion of the AA 961-…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmPumilio_B

   Plasmid

   #145945

   Purpose

   Insect Expression of DmPumilio

   Depositor

   Elisa Izaurralde

   Insert

   DmPumilio (pum Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   three silent and one non silent mutation and a de…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-lambdaN-HA-DmUPF1_92-1180-siRNAres-V5His6_N

   Plasmid

   #146990

   Purpose

   Insect Expression of DmUPF1_92-1180-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF1_92-1180-siRNAres (Upf1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   1 non silent mutations: G995E compared to the seq…

   Promoter

   Available Since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHS29

   Plasmid

   #171205

   Purpose

   bacterial expression for HIS purification of MEG-3 with mutations in the HMG-like motif

   Depositor

   Geraldine Seydoux

   Article

   Schmidt et al bioRxiv 2020.10.15.340570

   Insert

   meg-3 (meg-3 Nematode)
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   F705A,Y708A,Y713A, N725A

   Promoter

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C

   Plasmid

   #146013

   Purpose

   Insect Expression of DmSmaug_763-999

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug_763-999 (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   2 silent mutations and a deletion of the AA 961-9…

   Promoter

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-FLEX-SaCas9-U6-sgVamp1

   Plasmid

   #159919

   Purpose

   Mutagenesis of Vamp1

   Depositor

   Larry Zweifel

   Insert

   Vamp1 (Vamp1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

   Plasmid

   #159907

   Purpose

   Mutagenesis of Netrin1

   Depositor

   Larry Zweifel

   Insert

   Netrin1 (Ntn1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSD_SpCas9MT3-FKBP_SL

   Plasmid

   #107302

   Purpose

   Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   FKBP

   Expression

   Mammalian

   Mutation

   R1335K and fused to FKBP

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-7 asyn S129W

   Plasmid

   #105744

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Insert

   alpha-synuclein S129W (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S129W

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-7 asyn K32R

    Plasmid

    #105739

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein K32R (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K32R

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PTPRJ

    Plasmid

    #38888

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPRJ (PTPRJ Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    C1239S inactivating mutation

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pTH672-SUP35-Y351C

    Plasmid

    #29733

    Depositor

    Tobias von der Haar

    Insert

    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Y351C mutant gene of SUP35 including genomic sequ…

    Promoter

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. FC511

    Plasmid

    #27330

    Depositor

    Fred Cross

    Article

    Cross et al Yeast. 2011 Feb . 28(2):167-79.

    Insert

    CDC28 (CDC28 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    mutation CDC28-1N (P250L)

    Promoter

    Available Since

    Feb. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

14. Sub2-3 in pRS314

    Plasmid

    #90454

    Purpose

    yeast

    Depositor

    Christine Guthrie

    Article

    Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

    Insert

    SUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    sub2-3 mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sub2-4 in pRS314

    Plasmid

    #90455

    Purpose

    yeast

    Depositor

    Christine Guthrie

    Article

    Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

    Insert

    SUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    sub2-4 mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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16. Sub2-6 in pRS314

    Plasmid

    #90456

    Purpose

    yeast

    Depositor

    Christine Guthrie

    Article

    Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

    Insert

    SUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    sub2-6 mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-hM4D(Gi)-mCherry

    Plasmid

    #50475

    Purpose

    Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRES-H3.3-G34R-FLAG-BFP

    Plasmid

    #206140

    Purpose

    Expression of a FLAG tagged human histone mutant H3.3-G34R with BFP

    Depositor

    Maria Castro

    Insert

    H3.3 (H3-3A Human)
    

    Use

    Tags

    FLAG-eBFP

    Expression

    Mammalian

    Mutation

    G34R

    Promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6-MK1-EHMT2-V5

    Plasmid

    #31113

    Depositor

    Bernard Futscher

    Insert

    euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Stop codon mutated to Ala

    Promoter

    Available Since

    Aug. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-hChR2(H134R)-EYFP

    Plasmid

    #26973

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    hSyn

    Available Since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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