We narrowed to 14,849 results for: flag
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Plasmid#164832PurposemiHiFiCas9 expressing plasmidDepositorInsertmiHiFiCas9 (contains Brex27 at sequence 5031-5138).
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationchange R 691 to APromoterCMVAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3
Plasmid#165430PurposeExpresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.DepositorInsertFHS-paCaMKII(SD)
UseAAVTagsFHS(Flag, HisX6, Strep) tagExpressionMammalianMutationC450A, L514K, G528A, L531E, N538E: Light-insensit…PromoterCaMKII0.4Available SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_hUbC-dSpCas9-2xVP64-T2A-BSD
Plasmid#162333PurposeLentiviral expression of dSpCas9-2xVP64 with blasticidin resistanceDepositorInsertdSpCas9-2xVP64
UseLentiviralTagsFLAGExpressionMammalianMutationD10A and H840APromoterhUbCAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TP3
Plasmid#160086PurposeBacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKADepositorInsertTn5 transposase/APEX2 peroxidase fusion protein
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG646_lexO-HO_LexA-TF_LEU2MX
Plasmid#154815PurposeInducible expression of HO endonuclease in S. cerevisiaeDepositorInsertsTagsFLAGExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-nLight3.1
Plasmid#111061PurposeExpresses nLight3.1 in mammalian cellsDepositorInsertnLight3.1
TagsFlag tagExpressionMammalianAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-ALMS1
Plasmid#136828PurposeMammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tagDepositorInsertSNAP-ALMS1 (ALMS1 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep131
Plasmid#136826PurposeMammalian expression of the centrosomal protein Cep131 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep131 (CEP131 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)
Plasmid#128164PurposeUV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialPromoterUV5Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGHUC w-ERBIN (2-hybrid prey plasmid)
Plasmid#128163PurposeUV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).DepositorInsertERBIN PDZ domain (ERBIN Synthetic)
UseSynthetic BiologyTagsFLAG and Omega subunit of RNA polymeraseExpressionBacterialPromoterUV5Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.5
Plasmid#111058PurposeExpresses dLight1.5 in mammalian cellsDepositorInsertdLight1.5
TagsFlag tagExpressionMammalianAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
ANO10 (TMEM16KA-c001)
Plasmid#110267PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2.aBTX
Plasmid#122257PurposeExpress membrane-tethered alpha-bungarotoxin in Danio rerioDepositorInsertalpha-bungarotoxin
UseSynthetic BiologyTagsFLAGAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH243
Plasmid#121147PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeastDepositorInsertshumanized Streptococcus pyogenes Cas9
gRNA expression module
UseCRISPRTagsFlagExpressionYeastPromoterS.pombe rrk1 and nmt41Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PAX6 TALEN R
Plasmid#105525PurposeKnock in H2B-GFP into the human PAX6 locusDepositorInsertPax6 TALEN R
UseTALENTags3x FLAG and NLSExpressionMammalianPromoterCMVAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAX6 TALEN L
Plasmid#109034PurposeKnock in H2B-GFP into the human PAX6 locusDepositorInsertPax6 TALEN L
UseTALENTags3x FLAG and NLSExpressionMammalianPromoterCMVAvailable SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
e-ciCas9_pcDNA5
Plasmid#100553PurposeExpresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.DepositorInserte-ciCas9
UseCRISPR; Frt, flp-inTagsFLAGExpressionMammalianAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-VPS34
Plasmid#99327PurposeComponent for mammalian expression of recombinant PI3KC3-C1 complexDepositorAvailable SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-BECN1
Plasmid#99328PurposeComponent for mammalian expression of recombinant PI3KC3-C1 complexDepositorAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only