We narrowed to 11,627 results for: VARS

1. LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

   Plasmid

   #192370

   Purpose

   To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

   Plasmid

   #192374

   Purpose

   To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCStrunc-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAFMW-Nbr[K216E]

   Plasmid

   #188600

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   K216E [AAA=> GAA]

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAFMW-Nbr[C399-625 CM]

   Plasmid

   #188588

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler C-terminal domain, catalytic mutant
   

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   D435A [GAT=>GCC]; E435A [GAA=>GCC]

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAFMW-Nbr[C399-625]-Gwpep(Mm)

   Plasmid

   #188589

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
   

   Tags

   FLAG-Myc and GWpep mm

   Expression

   Insect

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAFMW-Nbr[K276A]

   Plasmid

   #188597

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   K276A [AAG=>GCG]

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAFMW-Nbr[C399-625]-Gwpep(Dm)

   Plasmid

   #188590

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler
   

   Tags

   FLAG-Myc and Gwpep dm

   Expression

   Insect

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAFMW-Gwpep(Dm)-Nbr[C399-625]

   Plasmid

   #188592

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
   

   Tags

   FLAG-Myc-Gwpep dm

   Expression

   Insect

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAFMW-Gwpep(Mm)-Nbr[C399-625]

   Plasmid

   #188591

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
   

   Tags

   FLAG-Myc-Gwpep mm

   Expression

   Insect

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAFMW-Nbr[R272A,K276A]

    Plasmid

    #188596

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R272A [CGG=> GCG], K276A [AAG=>GCG]

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAFMW-Nbr[K284A]

    Plasmid

    #188595

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K284A [AAG=> GCG]

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAFMW-Nbr[R272A]

    Plasmid

    #188594

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R272A [CGG=> GCG]

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAFMW-Nbr[K187A]

    Plasmid

    #188593

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K187A [AAA=> GCA]

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAFMW-Nbr

    Plasmid

    #188584

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

    Plasmid

    #158771

    Purpose

    Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)
    

    Use

    Lentiviral

    Mutation

    Flexible linkers, ADVVCC/QMSY cleavage site

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLeGO.sgGata1.4.RUNX1A.iG2

    Plasmid

    #181976

    Purpose

    gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

    Depositor

    Jan-Henning Klusmann

    Inserts

    GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
    RUNX1A (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFX-Sirius-KRasCT

    Plasmid

    #175831

    Purpose

    Express Sirius with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with plasma membrane-targeting sequence
    

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFX-SECFP-KRasCT

    Plasmid

    #175832

    Purpose

    Express SECFP with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    SECFP with plasma membrane-targeting sequence
    

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFX-iRFP-KRasCT

    Plasmid

    #175836

    Purpose

    Express iRFP with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with plasma membrane-targeting sequence
    

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSUMO human-cPLA2-C2-domain

    Plasmid

    #170752

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Human)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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