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pGY214

Plasmid

#239805

Purpose

Express EGFP and mRFP1 simultaneously in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

EGFP
mRFP1

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY219

Plasmid

#239806

Purpose

Express H2B-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H2B

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY220

Plasmid

#239807

Purpose

Express H3-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H3

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY224

Plasmid

#239808

Purpose

Express H2B-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H2B

Use

Fungal expression

Tags

mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY225

Plasmid

#239809

Purpose

Express H3-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H3

Use

Fungal expression

Tags

mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY232

Plasmid

#239810

Purpose

Express EGFP-P2A-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

EGFP
mRFP1

Use

Fungal expression

Tags

2A

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pNA-Y

Plasmid

#238993

Purpose

Node A carrying sgRNA-1 that can be used for the assembly of pEND-Y11

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

sgRNA-1

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AAVTRE3_smFP_HA

Plasmid

#201209

Purpose

AAV vector for TRE(TRE3G)-driven smFP_HA (HA-tagged spaghetti monster) expression

Depositor

Tetsuo Yamamori

Article

Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37

Insert

smFP _HA (addgene plasmid #59759)

Use

AAV

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-RMP64-FLAG (pAVA3889)

Plasmid

#239232

Purpose

Expresses C-FLAG tagged Rmp64 (CMV-RMP64-FLAG)

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

RMP64-FLAG

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-RMP24-FLAG (pAVA3890)

Plasmid

#239234

Purpose

Expresses C-FLAG tagged Rmp24 (CMV-RMP24-FLAG)

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

RMP24-FLAG

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-RPP21-FLAG (pAVA3892)

Plasmid

#239235

Purpose

Expresses C-FLAG tagged Rpp21 (CMV-RPP21-FLAG)

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

RPP21-FLAG

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-RPP25-FLAG (pAVA3895)

Plasmid

#239236

Purpose

Expresses C-FLAG tagged Rpp25 (CMV-RPP25-FLAG)

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

RPP25-FLAG

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-Z11

Plasmid

#239001

Purpose

Final circuit with -Ara: ON, +Ara: ON behaviour

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

Circuit with sfGFP expression (-Ara: ON, +Ara: ON)

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-KO-delVP1-VP1-588TAG

Plasmid

#240123

Purpose

Express AAV2-KO (R585A,R588A) capsid with ncAA mutant at site 588 of VP1

Depositor

Abhishek Chatterjee

Article

Pham et al bioRxiv 2024.07.20.604406

Inserts

RC2-DelVP1-R585,588A
VP1-588-TAG

Use

AAV

Expression

Mammalian

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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POC1426

Plasmid

#221573

Purpose

Plasmid containing the Insert 1 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1427

Plasmid

#221574

Purpose

Plasmid containing the Insert 2 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1428

Plasmid

#221575

Purpose

Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1429

Plasmid

#221576

Purpose

Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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P-Bf-fusA2

Plasmid

#235658

Purpose

Reports Cur activity in Bacteroides fragilis

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides fragilis fusA2 promoter

Mutation

The B. thetaiotaomicron fusA2 promoter lacking &q…

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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