We narrowed to 1,325 results for: pBAD
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Plasmid#119574PurposeLevel 0 part. PromoterDepositorInsertPBAD
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2m
Plasmid#105066PurposeA yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cellsDepositorInsertY-GECO2m
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVH004
Plasmid#80401PurposeContains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.DepositorUseSynthetic BiologyTagsC-terminal LZx domainExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVH015
Plasmid#80398PurposeContains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blotsDepositorUseSynthetic BiologyTagsC-terminal 3xFLAG tag and C-terminal LZx domainExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHYBAD94
Plasmid#42305DepositorInsertNpuDnaEC6
UseTagsGB1-H6ExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2s
Plasmid#105067PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2.1s
Plasmid#105068PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2.1s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
UseTagsExpressionBacterialMutationPromoterglnS and pBADAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCM001
Plasmid#124605PurposeExpresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pHDepositorInsertmCherry-pHluorin
UseTagsmCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…ExpressionBacterialMutationPromoterpDABAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas9cur
Plasmid#71538PurposeConstitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
Plasmid curing system
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWJB9
Plasmid#126650PurposeExpresses AdrA from a modified pLAFR3DepositorInsertadrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
UseTags8xHisExpressionBacterialMutationPromoterPBADAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15ABAD- YFP
Plasmid#186571Purposep15A ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterBAD promoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-8
Plasmid#163136PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterP(rhaB)Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6411
Plasmid#47050PurposeBglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coliDepositorInsertahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPbadAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the TM287/288 nanobody Nb_TM#2
Plasmid#132698Purposeexpresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISADepositorInsertnanobody Nb_TM#2
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCDF-NtXR1AtR2B2NtS1
Plasmid#160879PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: pBAD, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-MESVD/TRAP(RK)-C
Plasmid#61967PurposeDuet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVDDepositorInsertsN-mCherry-MESVD
TRAP(RK)-C-mCherry
UseTagsExpressionBacterialMutationPromoterPBAD and PLtetOAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLN43 - pBait-1xMS2-7GC[chiP-5']-TtrpA
Plasmid#222404Purposearabinose-induced synthesis of a hybrid RNA containing a 5' MS2 hairpin, E. coli chiP 5'UTR insert between XmaI and HindIII sites, surrounded by a 7bp GC clamp, and followed by a 3' trpA terminator.DepositorInsert5’UTR of chiP (-98 to +12, relative to AUG) (chiP E. coli)
UseTags1xMS2 hairpin, 7GC clamp, TtrpA terminatorExpressionBacterialMutationPromoterpBADAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAMC042z21
Plasmid#209747PurposeRepA(N99D) SC101 T0-BS7 proD SD8 sfGFP( V93AGGA Y151UAGA) ProK 5 tRNA operon tetA-orfL blaDepositorInsertsfGFP - tRNA operon
UseTagsExpressionBacterialMutationsfGFP - Valine 93 AGGA quadruplet codon, Tyrosine…PromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only